Abstract
Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha7 nicotinic ACh receptors. These data highlight the conundrum facing histologists--how 'real' is the labelling they see?
MeSH terms
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Animals
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Antibodies / immunology*
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Immunodominant Epitopes / metabolism
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Immunohistochemistry / methods
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Mice
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Mice, Knockout / metabolism
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Receptors, Nicotinic* / deficiency
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Receptors, Nicotinic* / immunology
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Receptors, Nicotinic* / metabolism
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alpha7 Nicotinic Acetylcholine Receptor
Substances
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Antibodies
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Chrna7 protein, mouse
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Immunodominant Epitopes
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Receptors, Nicotinic
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alpha7 Nicotinic Acetylcholine Receptor