Abstract
The vesicular monoamine transporter-2 (VMAT2) sequesters monoamine neurotransmitters into vesicles and prevents neurotoxicity. Human or monkey striatum generated three VMAT2 immunoreactive proteins of approximately 75 kDa, approximately 52-55 kDa, and approximately 45 kDa. The approximately 55-kDa band is considered the unglycosylated native protein. Deglycosylation of the VMAT2 from striatum or human VMAT2 expressed in HEK293 cells yielded a approximately 45-kDa, but not a 55-kDa immunoreactive band. We investigated this apparent mismatch between observed molecular size and predicted size.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analysis of Variance
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Animals
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Base Sequence
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Blotting, Western / methods
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Cloning, Molecular / methods
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Corpus Striatum / drug effects
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Corpus Striatum / metabolism*
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Dopamine / pharmacokinetics
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Glycosylation
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Humans
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Macaca mulatta
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Macromolecular Substances / metabolism
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Models, Molecular
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Molecular Weight
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Mutagenesis
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Phosphorylation
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Transfection
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Tritium / pharmacokinetics
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Vesicular Monoamine Transport Proteins / chemistry
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Vesicular Monoamine Transport Proteins / genetics
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Vesicular Monoamine Transport Proteins / metabolism*
Substances
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Macromolecular Substances
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Vesicular Monoamine Transport Proteins
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Tritium
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Dopamine