Abstract
In the globus pallidus, cannabinoid CB(1) receptors are localized pre-synaptically on GABAergic neurons. We assessed the influence of the endocannabinoids, anandamide, 2-arachidonoyl-glycerol (2-AG) and noladin ether, on the uptake of [(3)H]-GABA in pallidal slices from rat. Both 2-AG and noladin ether increased [(3)H]-GABA uptake (by 40.8 +/- 8.0% and 38.4 +/- 12.5%). The effect of 2-AG was blocked by the cannabinoid CB(1) receptor antagonist AM 251. In contrast, neither anandamide nor the agonist WIN 55,212-2 had an effect on [(3)H]-GABA uptake. Different roles might be played by different endocannabinoids, both physiologically and in basal ganglia disorders, such as Parkinson's disease.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonic Acids / pharmacology*
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Binding, Competitive / drug effects
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Cannabinoid Receptor Modulators / pharmacology*
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Endocannabinoids*
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Globus Pallidus / drug effects*
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Globus Pallidus / metabolism*
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Glycerides / pharmacology
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In Vitro Techniques
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Male
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Piperidines / pharmacology
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Polyunsaturated Alkamides
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Pyrazoles / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptor, Cannabinoid, CB1 / antagonists & inhibitors
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Tritium
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gamma-Aminobutyric Acid / pharmacokinetics*
Substances
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Arachidonic Acids
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Cannabinoid Receptor Modulators
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Endocannabinoids
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Glycerides
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Piperidines
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Polyunsaturated Alkamides
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Pyrazoles
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Receptor, Cannabinoid, CB1
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noladin ether
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Tritium
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AM 251
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gamma-Aminobutyric Acid
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glyceryl 2-arachidonate
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anandamide