The nonpsychoactive component of marijuana cannabidiol modulates chemotaxis and IL-10 and IL-12 production of murine macrophages both in vivo and in vitro

P Sacerdote; C Martucci; A Vaccani; F Bariselli; A E Panerai; A Colombo; D Parolaro; P Massi

doi:10.1016/j.jneuroim.2004.10.003

The nonpsychoactive component of marijuana cannabidiol modulates chemotaxis and IL-10 and IL-12 production of murine macrophages both in vivo and in vitro

J Neuroimmunol. 2005 Feb;159(1-2):97-105. doi: 10.1016/j.jneuroim.2004.10.003. Epub 2004 Dec 1.

Authors

P Sacerdote¹, C Martucci, A Vaccani, F Bariselli, A E Panerai, A Colombo, D Parolaro, P Massi

Affiliation

¹ Department of Pharmacology, via Vanvitelli 32, 20129 Milano, Italy. paola.sacerdote@unimi.it

PMID: 15652407
DOI: 10.1016/j.jneuroim.2004.10.003

Abstract

Cannabidiol is the main nonpsychoactive component of marijuana. We examined the ability of in vivo and in vitro cannabidiol to interfere with the production of interleukin (IL)-12 and IL-10 by murine macrophages and to modulate macrophage chemotaxis. Cannabidiol added in vitro to peritoneal macrophages significantly increased IL-12 and decreased IL-10 production. The CB1 and CB2 receptor antagonists prevented this modulation. Macrophages from animals treated with cannabidiol at the dose of 30 mg kg(-1) either orally or i.p. produced higher levels of IL-12 and lower levels of IL-10 in comparison to controls, and the CB receptor antagonists did not prevent these effects. Cannabidiol dose-dependently decreased fMLP-induced chemotaxis of macrophages, and the CB2 receptor antagonist prevented this decrease.

MeSH terms

Adjuvants, Immunologic / administration & dosage*
Adjuvants, Immunologic / metabolism
Adjuvants, Immunologic / pharmacology*
Administration, Oral
Animals
Camphanes / pharmacology
Cannabidiol / administration & dosage*
Cannabidiol / metabolism
Cannabidiol / pharmacology*
Cell Migration Inhibition
Cells, Cultured
Chemotaxis / drug effects*
Chemotaxis / immunology
Cytokines / biosynthesis
Down-Regulation / drug effects
Down-Regulation / immunology
Interleukin-10 / antagonists & inhibitors
Interleukin-10 / biosynthesis*
Interleukin-12 / biosynthesis*
Macrophage Activation / drug effects
Macrophage Activation / immunology
Macrophages, Peritoneal / drug effects*
Macrophages, Peritoneal / immunology
Macrophages, Peritoneal / metabolism
Male
Mice
Piperidines / pharmacology
Pyrazoles / pharmacology
Receptor, Cannabinoid, CB1 / antagonists & inhibitors
Receptor, Cannabinoid, CB1 / physiology
Receptor, Cannabinoid, CB2 / antagonists & inhibitors
Receptor, Cannabinoid, CB2 / physiology
Rimonabant
Up-Regulation / drug effects
Up-Regulation / immunology

Substances

Adjuvants, Immunologic
Camphanes
Cytokines
Piperidines
Pyrazoles
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
SR 144528
Interleukin-10
Interleukin-12
Cannabidiol
Rimonabant