Abstract
Evidence has accumulated that some of the angiotensin II AT1 receptor antagonists have insulin-sensitizing property. We thus examined the effect of telmisartan on insulin action using 3T3-L1 adipocytes. With standard differentiation inducers, a higher dose of telmisartan effectively facilitated differentiation of 3T3-L1 preadipocytes. Treatment of both differentiating adipocytes and fully differentiated adipocytes with telmisartan caused a dose-dependent increase in mRNA levels for PPARgamma target genes such as aP2 and adiponectin. By contrast, telmisartan attenuated 11beta-hydroxysteroid dehydrogenase type 1 mRNA level in differentiated adipocytes. Of note, we demonstrated for the first time that telmisartan augmented GLUT4 protein expression and 2-deoxy glucose uptake both in basal and insulin-stimulated state of adipocytes, which may contribute, at least partly, to its insulin-sensitizing ability.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Adipocytes / drug effects
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Adipocytes / metabolism
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Adipocytes / physiology*
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Angiotensin-Converting Enzyme Inhibitors / pharmacology
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Animals
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Benzimidazoles / pharmacology*
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Benzoates / pharmacology*
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Biological Transport / drug effects
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Deoxyglucose / pharmacology
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Gene Expression Regulation / drug effects
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Glucose / metabolism*
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Glucose Transporter Type 4
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Insulin / pharmacology
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Mice
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Monosaccharide Transport Proteins / drug effects
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Monosaccharide Transport Proteins / genetics*
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Muscle Proteins / drug effects
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Muscle Proteins / genetics*
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Receptor, Angiotensin, Type 1 / drug effects*
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Telmisartan
Substances
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Angiotensin-Converting Enzyme Inhibitors
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Benzimidazoles
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Benzoates
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Glucose Transporter Type 4
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Insulin
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Monosaccharide Transport Proteins
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Muscle Proteins
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Receptor, Angiotensin, Type 1
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Slc2a4 protein, mouse
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Deoxyglucose
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Glucose
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Telmisartan