Expression of a single dopamine transporter cDNA can confer two cocaine binding sites

J W Boja; L Markham; A Patel; G Uhl; M J Kuhar

doi:10.1097/00001756-199203000-00007

Expression of a single dopamine transporter cDNA can confer two cocaine binding sites

Neuroreport. 1992 Mar;3(3):247-8. doi: 10.1097/00001756-199203000-00007.

Authors

J W Boja¹, L Markham, A Patel, G Uhl, M J Kuhar

Affiliation

¹ Laboratory of Molecular Pharmacology, National Institute on Drug Abuse, Baltimore, MD.

PMID: 1515577
DOI: 10.1097/00001756-199203000-00007

Abstract

Radiolabeled cocaine analogs can bind to low and high affinity sites on striatal dopamine transporters (DAT). Recently, a cDNA encoding a rat brain dopamine transporter pDAT1 has been cloned. COS cells transfected with the pDAT1 in a eukaryotic expression vector express both a high (KD = 3.4 nM) and low affinity (KD = 163.6 nM) cocaine binding sites, suggesting that both sites are provided by a single gene product.

MeSH terms

Animals
Binding Sites / physiology
Carrier Proteins / genetics*
Cell Line
Cloning, Molecular
Cocaine* / analogs & derivatives*
Cocaine* / metabolism
Corpus Striatum / metabolism
DNA / biosynthesis*
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins*
Membrane Transport Proteins*
Membranes / metabolism
Nerve Tissue Proteins / genetics*
Radioligand Assay
Rats
Receptors, Drug / genetics*

Substances

Carrier Proteins
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins
Membrane Transport Proteins
Nerve Tissue Proteins
Receptors, Drug
Slc6a3 protein, rat
cocaine receptor
(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
DNA
Cocaine