Abstract
The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CHO Cells
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COS Cells
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Carrier Proteins / metabolism
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Cell Membrane / metabolism
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Cell-Penetrating Peptides
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Chlorocebus aethiops
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Cricetinae
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Cricetulus
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Cytoplasm / metabolism
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Disulfides / metabolism
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Fibroblasts / metabolism
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Galanin
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Gene Silencing
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Gene Transfer Techniques*
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Genes, Reporter
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Green Fluorescent Proteins
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Luciferases / metabolism
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Luminescent Proteins / metabolism
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Mice
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Peptide Fragments / metabolism
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RNA, Small Interfering / genetics*
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RNA, Small Interfering / metabolism
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Recombinant Fusion Proteins / metabolism
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Transgenes
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Wasp Venoms
Substances
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Carrier Proteins
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Cell-Penetrating Peptides
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Disulfides
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Luminescent Proteins
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Peptide Fragments
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RNA, Small Interfering
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Recombinant Fusion Proteins
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Wasp Venoms
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transportan
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Green Fluorescent Proteins
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Galanin
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penetratin
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Luciferases