Discrimination between two types of P2 purinoceptors by suramin in rat hepatocytes

Eur J Pharmacol. 1992 Aug 3;226(4):363-5. doi: 10.1016/0922-4106(92)90054-y.

Abstract

Suramin, currently reported as a P2 purinoceptor antagonist, competitively inhibited P2 purinoceptor agonist-induced phospholipase C (PLC) stimulation, but not P2 purinoceptor agonist-induced adenylate cyclase (AC) inhibition in rat hepatocytes. Suramin did not inhibit vasopressin-induced PLC activity. We conclude that there are two types of P2 purinoceptors; one is suramin-sensitive and coupled to PLC in a stimulatory manner, and the other is suramin-insensitive and coupled to AC in an inhibitory manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclase Inhibitors
  • Animals
  • Binding Sites
  • Liver / cytology
  • Liver / drug effects*
  • Liver / enzymology
  • Male
  • Purinergic Antagonists
  • Rats
  • Rats, Wistar
  • Receptors, Purinergic / drug effects*
  • Suramin / pharmacology*
  • Type C Phospholipases / metabolism
  • Vasopressins / pharmacology

Substances

  • Adenylyl Cyclase Inhibitors
  • Purinergic Antagonists
  • Receptors, Purinergic
  • Vasopressins
  • Suramin
  • Type C Phospholipases