Abstract
The distribution of histamine H1, H3, dopamine D1 and D2 receptors in the brain was studied by receptor autoradiography using a high-sensitivity and high-resolution imaging plate system. [3H]Pyrilamine, [3H](R)alpha-methyl-histamine, [11C]SCH23390, and [11C]N-methylspiperone (or [11C]YM-09151-2) were used as ligands to identify H1, H3, D1 and D2 receptors, respectively. Two different receptors (dopamine D2 and histamine H3) could be also labelled simultaneously in a single cryostat-sliced section using [11C]N-methylspiperone and [3H](R)alpha-methylhistamine, respectively. The imaging plate system is useful for receptor autoradiography of positron emitter-labelled and tritium-labelled receptor-ligands because of its high sensitivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoradiography
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Brain / diagnostic imaging
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Dopamine Agents
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Dopamine D2 Receptor Antagonists
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Guinea Pigs
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Histamine Agonists
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Histamine Antagonists
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Ligands
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Male
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Methylhistamines
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Rats
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Rats, Wistar
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Receptors, Cell Surface / antagonists & inhibitors
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Receptors, Cell Surface / metabolism*
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Receptors, Dopamine D1 / antagonists & inhibitors
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Receptors, Dopamine D1 / metabolism
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Receptors, Dopamine D2 / metabolism
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Receptors, Histamine / metabolism
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Receptors, Histamine H3
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Spiperone / analogs & derivatives
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Tomography, Emission-Computed
Substances
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Dopamine Agents
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Dopamine D2 Receptor Antagonists
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Histamine Agonists
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Histamine Antagonists
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Ligands
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Methylhistamines
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Receptors, Cell Surface
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Receptors, Dopamine D1
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Receptors, Dopamine D2
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Receptors, Histamine
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Receptors, Histamine H3
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Spiperone
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alpha-methylhistamine
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3-N-methylspiperone