Abstract
Three subtypes of alpha 2 adrenergic receptors have been identified in the human and rat. The subtype located on human chromosome 2 (alpha 2-C2) is unique in that it is expressed mainly in the peripheral tissues and lacks sites for N-linked glycosylation. We isolated the gene encoding the mouse homolog of the human alpha 2-C2 adrenergic receptor (M alpha 2-2H). The deduced amino acid sequence of the M alpha 2-2H shows 82% and 96% identity to the human alpha 2-C2 and the rat RNG alpha 2 adrenergic receptors, respectively. Southern blot analysis demonstrated that the M alpha 2-2H was encoded by a single copy gene and was distinct from the mouse homologs of the alpha 2-C4 and alpha 2-C10 adrenergic receptors. When expressed in COS-7 cells, the M alpha 2-2H exhibited a pharmacological profile similar to the human alpha 2-C2 and rat RNG alpha 2 receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic beta-Agonists / metabolism
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Adrenergic beta-Antagonists / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Cell Line
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Humans
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Mice
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Molecular Sequence Data
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Rats
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Receptors, Adrenergic, alpha / biosynthesis
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Receptors, Adrenergic, alpha / genetics*
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Receptors, Adrenergic, alpha / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Adrenergic beta-Agonists
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Adrenergic beta-Antagonists
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Receptors, Adrenergic, alpha
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Recombinant Proteins
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DNA
Associated data
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GENBANK/D10916
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GENBANK/D10917
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GENBANK/D10918
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GENBANK/D10919
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GENBANK/D10920
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GENBANK/L00979
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GENBANK/S39218
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GENBANK/S39219
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GENBANK/S39220
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GENBANK/S39221