Abstract
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Cloning, Molecular
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Gene Library
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Humans
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Methylcholanthrene / pharmacology
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Mice
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Molecular Sequence Data
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Oligonucleotide Probes
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Polychlorinated Dibenzodioxins / metabolism
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Polymerase Chain Reaction
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Receptors, Aryl Hydrocarbon
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Receptors, Drug / genetics*
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Recombinant Fusion Proteins / metabolism
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Oligonucleotide Probes
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Polychlorinated Dibenzodioxins
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Receptors, Aryl Hydrocarbon
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Receptors, Drug
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Recombinant Fusion Proteins
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Methylcholanthrene
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Chloramphenicol O-Acetyltransferase