Abstract
Apoptosis in human umbilical vein endothelial cells (HUVECs) was prevented by transfection with the gene for the human full-length peroxisome proliferator-activated receptor alpha (PPARalpha), or acyl-coenzyme A synthetase (AcylCS) into HUVECs. In contrast, ligands/activators of PPARgamma 1 induced apoptosis by a cytochrome c-dependent mechanism in HUVECs transfected with human full-length PPARgamma 1, but not in hepatocytes. Co-transfection of PPARgamma 1 and PPARalpha protected the HUVEC apoptosis. The results suggest that the apoptosis of endothelial cells may be mediated by genes of PPARgamma1 and PPARalpha.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / genetics*
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Apoptosis / physiology
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Cells, Cultured
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Coenzyme A Ligases / genetics
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Coenzyme A Ligases / metabolism
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Endothelium, Vascular / cytology*
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Endothelium, Vascular / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Hepatocytes / cytology
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Hepatocytes / metabolism
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Humans
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Lipid Metabolism
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transfection
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Umbilical Veins
Substances
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Escherichia coli Proteins
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Coenzyme A Ligases
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acyl-coenzyme A synthetase, E coli