Yeast assays for G-protein-coupled receptors

Recept Channels. 2002;8(5-6):343-52.

Abstract

Yeast assays for G-protein-coupled receptors have many attractions due to their simplicity, low cost, and lack of endogenous receptors. Since the first report of functional coupling of the human beta 2 adrenergic receptor to the yeast pheromone-response pathway in 1990, the technology has developed to a point at which more than 30 heterologous GPCRs are now published to couple. Major breakthroughs have come from an understanding of receptor-G protein interactions, alongside advances in knowledge of the structure of heterotrimeric G proteins. Yeast screens have been used to identify ligands both from compound collections and through the autocrine expression of peptide libraries. Yeast genetics has also been applied to a functional analysis of GPCRs and peptide ligands. In this review we describe the historical development of yeast GPCR assay systems and their current applications.

Publication types

  • Review

MeSH terms

  • Biochemistry / methods*
  • Biotechnology / methods
  • GTP-Binding Protein alpha Subunits*
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • GTP-Binding Protein beta Subunits*
  • GTP-Binding Protein gamma Subunits*
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / metabolism*
  • Heterotrimeric GTP-Binding Proteins / chemistry
  • Humans
  • Ligands
  • Melatonin / pharmacology
  • Models, Biological
  • Models, Molecular
  • Peptides / chemistry
  • Pheromones / metabolism
  • Protein Binding
  • Protein Conformation
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / metabolism*
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Receptors, Melatonin
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry

Substances

  • GTP-Binding Protein alpha Subunits
  • GTP-Binding Protein beta Subunits
  • GTP-Binding Protein gamma Subunits
  • Ligands
  • Peptides
  • Pheromones
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Melatonin
  • STE18 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Ste4 protein, S cerevisiae
  • GTP-Binding Proteins
  • GPA1 protein, S cerevisiae
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • Heterotrimeric GTP-Binding Proteins
  • Melatonin