Abstract
The effect of cannabinoid on the tyrosine phosphorylation of focal adhesion kinase (FAK) and focal adhesion kinase-related non-kinase (FRNK) was investigated in differentiated mouse neuroblastoma N1E-115 cells. HU-210, a potent cannabinoid agonist, elicited a time-dependent enhancement of tyrosine phosphorylation of FRNK, but not FAK. Pretreatment of cells with antisense oligodeoxynucleotide targeting CB1 cannabinoid receptor abolished HU-210-induced FRNK tyrosine phosphorylation. In addition, pretreatment of cells with 8-Br-cAMP also blocked HU-210-induced FRNK tyrosine phosphorylation. These data demonstrated that HU-210 induces FRNK tyrosine phosphorylation by activating G(i)-coupled CB1 cannabinoid receptor in N1E-115 cells. This newly discovered, cannabinoid-induced FRNK tyrosine phosphorylation might be a novel mechanism for cannabinoid-induced functional changes.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Animals
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Cannabinoids / pharmacology
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Dronabinol / analogs & derivatives
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Dronabinol / pharmacology
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Enzyme Activators / pharmacology
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Mice
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Neuroblastoma / drug therapy
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Neuroblastoma / metabolism*
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Oligonucleotides, Antisense / pharmacology
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Phosphorylation / drug effects
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Protein-Tyrosine Kinases / metabolism*
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RNA, Messenger / antagonists & inhibitors
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Receptors, Cannabinoid
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Receptors, Drug / antagonists & inhibitors
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Receptors, Drug / genetics
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Receptors, Drug / metabolism*
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Tumor Cells, Cultured
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Tyrosine / metabolism
Substances
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Cannabinoids
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Enzyme Activators
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Oligonucleotides, Antisense
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RNA, Messenger
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Receptors, Cannabinoid
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Receptors, Drug
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8-Bromo Cyclic Adenosine Monophosphate
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Tyrosine
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Dronabinol
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FAK-related nonkinase
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Protein-Tyrosine Kinases
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Ptk2 protein, mouse
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HU 211