Abstract
Adenosine is a putative sleep factor with effects mainly mediated by the A1 receptor. Recent studies have implicated the hypothalamic orexin/hypocretin-containing neurons in the control of sleep-wakefulness. To help determine if adenosine might play a role in the control of orexin neurons, immunohistochemistry was used to characterize the distribution of adenosine A1 receptor protein on the orexinergic neurons. About 30% of orexin-containing neurons were labeled. The data supports the presence of adenosine A1 receptors on orexinergic neurons and suggests a possible substrate for a functional role of adenosine in the regulation of orexinergic activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylcholine / metabolism
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Adenosine / metabolism*
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Animals
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Basal Nucleus of Meynert / metabolism
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Carrier Proteins / metabolism*
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Cell Count
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Fluorescent Antibody Technique
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Hypothalamus / cytology
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Hypothalamus / metabolism*
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Intracellular Signaling Peptides and Proteins*
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Male
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Neural Inhibition / physiology
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Neurons / cytology
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Neurons / metabolism*
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Neuropeptides / metabolism*
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Orexin Receptors
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Orexins
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Presynaptic Terminals / metabolism
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Presynaptic Terminals / ultrastructure
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Rats
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Rats, Sprague-Dawley
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide
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Receptors, Purinergic P1 / metabolism*
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Sleep / physiology*
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Wakefulness / physiology*
Substances
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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Neuropeptides
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Orexin Receptors
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Orexins
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide
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Receptors, Purinergic P1
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Adenosine
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Acetylcholine