Abstract
The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / metabolism
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Axons / ultrastructure
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Calbindins
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Cell Size / physiology
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Colon / cytology
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Colon / innervation
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Colon / metabolism
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Female
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Guinea Pigs
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Ileum / cytology
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Ileum / innervation
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Ileum / metabolism
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Immunohistochemistry
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Intestinal Mucosa / metabolism
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Intestines / cytology
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Intestines / innervation*
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Male
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Microscopy, Confocal
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Myenteric Plexus / cytology
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Myenteric Plexus / metabolism*
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Neurons, Afferent / cytology
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Neurons, Afferent / metabolism*
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Nociceptors / cytology
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Nociceptors / metabolism*
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Pain / metabolism*
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Pain / physiopathology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, Drug / genetics
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Receptors, Drug / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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S100 Calcium Binding Protein G / metabolism
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Sequence Homology, Nucleic Acid
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Visceral Afferents / cytology
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Visceral Afferents / metabolism*
Substances
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Calbindins
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RNA, Messenger
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Receptors, Drug
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S100 Calcium Binding Protein G