Abstract
Recent studies from our laboratory have shown that substance P can elicit transcription factor activation in dendritic cells. In the present study, we extend these findings by demonstrating the presence of authentic substance P (NK-1) receptors on both normal murine and human dendritic cells. Specifically, we demonstrate the presence of mRNA encoding NK-1 tachykinin receptors and have utilized specific antibodies to detect the expression of NK-1 receptor protein in dendritic cells by Western blot analysis and flow cytometry. These data provide a crucial first step in determining the potential of substance P to modulate dendritic cell function.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Cells, Cultured
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Dendritic Cells / chemistry*
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Flow Cytometry
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Fluorescent Antibody Technique
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Gene Expression / immunology
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Humans
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Integrin alphaXbeta2 / analysis
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Pancreas / cytology
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RNA, Messenger / analysis
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Rats
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Receptors, Neurokinin-1 / analysis*
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Receptors, Neurokinin-1 / genetics*
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Receptors, Neurokinin-1 / immunology
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Transcription, Genetic / immunology
Substances
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Integrin alphaXbeta2
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RNA, Messenger
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Receptors, Neurokinin-1