Expression of authentic substance P receptors in murine and human dendritic cells

J Neuroimmunol. 2001 Mar 1;114(1-2):131-41. doi: 10.1016/s0165-5728(00)00466-5.

Abstract

Recent studies from our laboratory have shown that substance P can elicit transcription factor activation in dendritic cells. In the present study, we extend these findings by demonstrating the presence of authentic substance P (NK-1) receptors on both normal murine and human dendritic cells. Specifically, we demonstrate the presence of mRNA encoding NK-1 tachykinin receptors and have utilized specific antibodies to detect the expression of NK-1 receptor protein in dendritic cells by Western blot analysis and flow cytometry. These data provide a crucial first step in determining the potential of substance P to modulate dendritic cell function.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Dendritic Cells / chemistry*
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Gene Expression / immunology
  • Humans
  • Integrin alphaXbeta2 / analysis
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Pancreas / cytology
  • RNA, Messenger / analysis
  • Rats
  • Receptors, Neurokinin-1 / analysis*
  • Receptors, Neurokinin-1 / genetics*
  • Receptors, Neurokinin-1 / immunology
  • Transcription, Genetic / immunology

Substances

  • Integrin alphaXbeta2
  • RNA, Messenger
  • Receptors, Neurokinin-1