Abstract
Syk has been implicated in activated immunoreceptors to downstream signaling events in hematopoietic cells. Here we report that Syk is expressed in neuron-like cells and involved in neuron-like differentiation of embryonal carcinoma P19 cells. Immunoblot, RT-PCR, and Northern analysis indicated that Syk is expressed in mouse brain, PC12 and P19 cells. In addition, Syk was found to be tyrosine phosphorylated during neuron-like differentiation of P19 cells. Furthermore, adenovirus-mediated overexpression of Syk induced supernumerary neurite formation and extracellular signal-regulated kinase (ERK) activation in P19 cells. These results suggest that Syk plays an important role in signaling steps leading to ERK activation in P19 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Animals
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Brain / cytology
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Brain / enzymology
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Carcinoma, Embryonal / enzymology*
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Carcinoma, Embryonal / pathology
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Cell Differentiation*
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DNA, Recombinant
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Enzyme Activation
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Enzyme Precursors / genetics
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Enzyme Precursors / metabolism*
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Gene Expression Regulation, Enzymologic
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Intracellular Signaling Peptides and Proteins
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Mice
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Mitogen-Activated Protein Kinases / metabolism
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Neurites / metabolism
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Neurons / cytology*
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Neurons / enzymology
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Phosphorylation
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Syk Kinase
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Tumor Cells, Cultured
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Tyrosine / metabolism
Substances
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DNA, Recombinant
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Enzyme Precursors
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Intracellular Signaling Peptides and Proteins
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RNA, Messenger
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Tyrosine
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Protein-Tyrosine Kinases
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Syk Kinase
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Syk protein, mouse
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Mitogen-Activated Protein Kinases