Abstract
Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE-V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT--PCR) demonstrated mRNA for PDE-V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM - 10 microM; pEC(50)=6.7+/-0.3) was potentiated significantly by the specific inhibitor of PDE-V, 4-[[3,4-(methylenedioxy)benzyl]amino]-6-chloroquinazoline (MBCQ; 1 microM; pEC(50)=10.5+/-0.04). These data show that PDE-V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide-evoked vasorelaxation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3',5'-Cyclic-GMP Phosphodiesterases
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Animals
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Arteries / drug effects
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Arteries / enzymology*
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Arteries / physiology*
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Blotting, Western
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Cyclic Nucleotide Phosphodiesterases, Type 5
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Immunohistochemistry
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In Vitro Techniques
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Male
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Mesenteric Arteries / drug effects
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Mesenteric Arteries / physiology
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Muscle Relaxation / drug effects
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Muscle, Smooth, Vascular / drug effects
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Nitric Oxide Donors / pharmacology
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Phosphoric Diester Hydrolases / biosynthesis*
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Phosphoric Diester Hydrolases / physiology*
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Precipitin Tests
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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Vascular Resistance / drug effects
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Vascular Resistance / physiology*
Substances
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Nitric Oxide Donors
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RNA, Messenger
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Phosphoric Diester Hydrolases
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3',5'-Cyclic-GMP Phosphodiesterases
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Cyclic Nucleotide Phosphodiesterases, Type 5
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Pde5a protein, rat