Upregulation of P53 protein in rat heart subjected to a transient occlusion of the coronary artery followed by reperfusion

Z Xie; T Koyama; K Abe; Y Fuji; H Sawa; K Nagtashima

doi:10.2170/jjphysiol.50.159

Upregulation of P53 protein in rat heart subjected to a transient occlusion of the coronary artery followed by reperfusion

Jpn J Physiol. 2000 Feb;50(1):159-62. doi: 10.2170/jjphysiol.50.159.

Authors

Z Xie¹, T Koyama, K Abe, Y Fuji, H Sawa, K Nagtashima

Affiliation

¹ 3rd Department of Anatomy, School of Medicine, Hokkaido University, Sapporo, 064-0821 Japan.

PMID: 10866709
DOI: 10.2170/jjphysiol.50.159

Abstract

The accumulation of p53 protein in cardiomyocyte nuclei was immunohistochemically demonstrated by the pronounced staining of p53 antibody in 4 h-reperfused ventricular tissue after a 45-min coronary occlusion. The occurrence of apoptosis in the reperfused ventricular tissue was evidenced by positive TUNEL staining and DNA laddering on agarose gels.

MeSH terms

Animals
Apoptosis / physiology
Coronary Circulation / physiology*
Heart Ventricles / metabolism
Heart Ventricles / pathology
In Situ Nick-End Labeling
Male
Muscle Fibers, Skeletal / metabolism
Muscle Fibers, Skeletal / pathology
Myocardial Ischemia / metabolism*
Myocardial Ischemia / pathology
Myocardial Reperfusion Injury / metabolism*
Myocardial Reperfusion Injury / pathology
Myocardium / metabolism*
Myocardium / pathology
Rats
Rats, Wistar
Tumor Suppressor Protein p53 / metabolism*

Substances

Tumor Suppressor Protein p53