Abstract
Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both transiently elevated [Ca(2+)](i). A subsequent addition of Ca(2+) into the bathing medium caused a second [Ca(2+)](i) change which was blocked by lanthanum (50 microM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca(2+) entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca(2+) entry in excitable cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
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Aniline Compounds / metabolism
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Animals
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Calcium / metabolism*
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Calcium / pharmacology
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Calcium Signaling / drug effects
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Calcium-Transporting ATPases / antagonists & inhibitors
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Calcium-Transporting ATPases / metabolism
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Carbachol / pharmacology
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Cells, Cultured
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Cholinergic Agonists / pharmacology
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Electric Conductivity
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Excitatory Amino Acid Antagonists / pharmacology
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Hippocampus / cytology*
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Hippocampus / metabolism
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Lanthanum / pharmacology
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Neurons / drug effects
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Neurons / metabolism*
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Potassium / pharmacology
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Rats
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Tetrodotoxin / pharmacology
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Thapsigargin / pharmacology
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Xanthenes / metabolism
Substances
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Aniline Compounds
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Cholinergic Agonists
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Excitatory Amino Acid Antagonists
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Xanthenes
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Fluo-3
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Tetrodotoxin
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Thapsigargin
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Lanthanum
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6-Cyano-7-nitroquinoxaline-2,3-dione
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Carbachol
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Calcium-Transporting ATPases
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Potassium
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Calcium