[(35)S]-GTPgammaS autoradiography reveals alpha(2) adrenoceptor-mediated G-protein activation in amygdala and lateral septum

Neuropharmacology. 2000 Apr 3;39(6):1111-3. doi: 10.1016/s0028-3908(99)00235-x.

Abstract

alpha(2)-adrenoceptor-mediated G-protein activation was examined by [(35)S]-GTPgammaS autoradiography. In alpha(2)-adrenoceptor-rich regions (amygdala, lateral septum), noradrenaline stimulated [(35)S]-GTPgammaS binding. These actions were abolished by the selective alpha(2) antagonist, atipamezole. Conversely, in caudate nucleus, which expresses few alpha(2) receptors, noradrenaline-induced stimulation was not inhibited by atipamezole, suggesting that it is not mediated by alpha(2)-adrenoceptors.

MeSH terms

  • Adrenergic Antagonists / pharmacology
  • Amygdala / metabolism*
  • Animals
  • Autoradiography
  • GTP-Binding Proteins / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism*
  • Imidazoles / pharmacology
  • In Vitro Techniques
  • Norepinephrine / pharmacology
  • Rats
  • Receptors, Adrenergic, alpha-2 / drug effects
  • Receptors, Adrenergic, alpha-2 / metabolism
  • Receptors, Adrenergic, alpha-2 / physiology*
  • Septum of Brain / metabolism*
  • Sulfur Radioisotopes

Substances

  • Adrenergic Antagonists
  • Imidazoles
  • Receptors, Adrenergic, alpha-2
  • Sulfur Radioisotopes
  • atipamezole
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • GTP-Binding Proteins
  • Norepinephrine