Abstract
alpha(2)-adrenoceptor-mediated G-protein activation was examined by [(35)S]-GTPgammaS autoradiography. In alpha(2)-adrenoceptor-rich regions (amygdala, lateral septum), noradrenaline stimulated [(35)S]-GTPgammaS binding. These actions were abolished by the selective alpha(2) antagonist, atipamezole. Conversely, in caudate nucleus, which expresses few alpha(2) receptors, noradrenaline-induced stimulation was not inhibited by atipamezole, suggesting that it is not mediated by alpha(2)-adrenoceptors.
MeSH terms
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Adrenergic Antagonists / pharmacology
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Amygdala / metabolism*
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Animals
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Autoradiography
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GTP-Binding Proteins / metabolism*
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism*
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Imidazoles / pharmacology
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In Vitro Techniques
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Norepinephrine / pharmacology
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Rats
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Receptors, Adrenergic, alpha-2 / drug effects
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Receptors, Adrenergic, alpha-2 / metabolism
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Receptors, Adrenergic, alpha-2 / physiology*
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Septum of Brain / metabolism*
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Sulfur Radioisotopes
Substances
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Adrenergic Antagonists
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Imidazoles
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Receptors, Adrenergic, alpha-2
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Sulfur Radioisotopes
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atipamezole
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Guanosine 5'-O-(3-Thiotriphosphate)
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GTP-Binding Proteins
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Norepinephrine