Abstract
We have investigated the alpha(1A)-adrenoceptor-mediated activation of 46 and 54 kDa isoforms of c-jun N-terminal kinase (JNK) and of p38 mitogen-activated protein kinase. The alpha(1)-adrenoceptor agonist phenylephrine activated all three kinases but with different time courses and maximal effects. Activation of all three kinases was insensitive to the phosphatidylinositol-3-kinase inhibitor wortmannin but was enhanced by the protein kinase C inhibitor bisindolylmaleimide I; a protein kinase C-activating phorbol ester inhibited JNK but not p38 activation. Activation of 54 kDa JNK, but not of the other two kinases, was inhibited by pertussis toxin and the phospholipase C inhibitor U 73,122. We conclude that alpha(1)-adrenoceptor stimulation activates 46 kDa JNK, 54 kDa JNK and p38 but uses at least partly different pathways to do so.
Copyright 1999 Academic Press.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adrenergic alpha-Agonists / pharmacology
-
Adrenergic alpha-Antagonists / pharmacology
-
Androstadienes / pharmacology
-
Animals
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
-
Enzyme Activation
-
Enzyme Inhibitors / pharmacology
-
Estrenes / pharmacology
-
Gene Expression
-
Humans
-
Indoles / pharmacology
-
JNK Mitogen-Activated Protein Kinases
-
Kinetics
-
Maleimides / pharmacology
-
Mitogen-Activated Protein Kinases*
-
Pertussis Toxin
-
Phenylephrine / pharmacology
-
Phosphoinositide-3 Kinase Inhibitors
-
Prazosin / pharmacology
-
Protein Kinase C / metabolism
-
Pyrrolidinones / pharmacology
-
Rats
-
Receptors, Adrenergic, alpha-1 / genetics
-
Receptors, Adrenergic, alpha-1 / metabolism*
-
Tetradecanoylphorbol Acetate / pharmacology
-
Transfection
-
Type C Phospholipases / antagonists & inhibitors
-
Virulence Factors, Bordetella / pharmacology
-
Wortmannin
-
p38 Mitogen-Activated Protein Kinases
Substances
-
ADRA1A protein, human
-
Adra1a protein, rat
-
Adrenergic alpha-Agonists
-
Adrenergic alpha-Antagonists
-
Androstadienes
-
Enzyme Inhibitors
-
Estrenes
-
Indoles
-
Maleimides
-
Phosphoinositide-3 Kinase Inhibitors
-
Pyrrolidinones
-
Receptors, Adrenergic, alpha-1
-
Virulence Factors, Bordetella
-
1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
-
Phenylephrine
-
Pertussis Toxin
-
Protein Kinase C
-
Calcium-Calmodulin-Dependent Protein Kinases
-
JNK Mitogen-Activated Protein Kinases
-
Mitogen-Activated Protein Kinases
-
p38 Mitogen-Activated Protein Kinases
-
Type C Phospholipases
-
bisindolylmaleimide
-
Tetradecanoylphorbol Acetate
-
Prazosin
-
Wortmannin