Abstract
Various sigma (sigma) ligands including (+)-pentazocine stimulated [35S]GTPgammaS binding in synaptic membranes from the mouse cerebellum. The (+)-pentazocine-stimulated [35S]GTPgammaS binding was blocked by the treatment of membranes with pertussis toxin (PTX), but completely recovered by the reconstitution of PTX-treated membranes with recombinant Gi1, but not with GoA. These findings suggest that metabotropic sigma receptors are selectively coupled to Gi1 protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / pharmacology
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Brain Chemistry*
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Dimerization
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GTP-Binding Protein alpha Subunits, Gi-Go / metabolism*
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
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Macromolecular Substances
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Male
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Mice
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Mice, Mutant Strains
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Pentazocine / pharmacology
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Pertussis Toxin
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Receptors, sigma / metabolism*
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Recombinant Proteins / metabolism*
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Sulfur Radioisotopes / metabolism
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Synaptic Membranes / drug effects
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Synaptic Membranes / metabolism
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Virulence Factors, Bordetella / pharmacology
Substances
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Bacterial Proteins
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Macromolecular Substances
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Receptors, sigma
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Recombinant Proteins
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Sulfur Radioisotopes
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Virulence Factors, Bordetella
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Guanosine 5'-O-(3-Thiotriphosphate)
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Pertussis Toxin
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GTP-Binding Protein alpha Subunits, Gi-Go
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Pentazocine