Abstract
The regulation of striatal preprotachykinin (PPT) mRNA expression can be mediated through both dopamine (DA) D1 and serotonin (5-HT) 5-HT2A/2C receptors. In the present study, we used in situ hybridization to examine possible synergistic interactions between 5-HT2A/2C and D1 receptor-mediated regulation of striatal PPT mRNA levels in the rat depleted of DA with 6-hydroxydopamine. Acute administration of the 5-HT2A/2C receptor agonist DOI (2 mg/kg) significantly increased (+75%) PPT mRNA levels in the dorsal striatum. Acute administration of the D1 receptor agonist SKF-38393 (2 mg/kg) did not significantly alter PPT mRNA levels in the dorsal striatum. However, the co-administration of SKF-38393 and DOI produced a significant increase (+300%) in striatal PPT mRNA expression restricted to the periventricular region of the dorsal-medial striatum. This synergistic interaction was not observed in the remaining aspect of the dorsal striatum where DOI alone increased PPT mRNA expression. These data show that 5-HT2A/2C and D1 receptors can act in a synergistic manner to regulate striatal PPT mRNA in a subregion of the DA-depleted striatum.
Copyright 1999 Elsevier Science B.V.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology*
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Amphetamines / pharmacology*
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Animals
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Chromatography, High Pressure Liquid
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Corpus Striatum / drug effects*
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Corpus Striatum / metabolism
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Denervation
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Dopamine / pharmacology*
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Dopamine Agonists / pharmacology*
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Drug Synergism
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Gene Expression Regulation / drug effects*
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In Situ Hybridization
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Male
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / genetics
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Neurons / drug effects
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Neurons / metabolism
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Oxidopamine / toxicity*
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Parkinson Disease, Secondary / chemically induced
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Protein Precursors / biosynthesis*
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Protein Precursors / genetics
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RNA, Messenger / biosynthesis*
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Rats
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Rats, Sprague-Dawley
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2C
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Receptors, Dopamine D1 / drug effects
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Receptors, Dopamine D1 / physiology*
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Receptors, Serotonin / drug effects
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Receptors, Serotonin / physiology*
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Serotonin / pharmacology*
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Serotonin Receptor Agonists / pharmacology*
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Tachykinins / biosynthesis*
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Tachykinins / genetics
Substances
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Amphetamines
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Dopamine Agonists
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Nerve Tissue Proteins
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Protein Precursors
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RNA, Messenger
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2C
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Receptors, Dopamine D1
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Receptors, Serotonin
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Serotonin Receptor Agonists
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Tachykinins
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preprotachykinin
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Serotonin
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2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
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Oxidopamine
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4-iodo-2,5-dimethoxyphenylisopropylamine
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Dopamine