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HPLC Determination of Lovastatin in Rat Tissue

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Abstract

A simple HPLC method has been developed and validated for the determination of lovastatin in rat tissues. Samples were prepared by a simple protein precipitation. Separation was carried out on a C18 column with a mobile phase of acetonitrile:0.05 M ammonium acetate, a flow rate of 1.0 mL min−1 and with detection at 238 nm. There was no interference from endogenous tissue compounds. The calibration curve was linear from 0.0175 to 7.0 μg mL−1 with a limit of detection of 0.006 μg mL−1. The method was used to measure the concentration of lovastatin in rat tissue after a single oral dose. The highest level was observed in the liver, then in kidney, heart and spleen; the lowest level was found in the brain. These results suggest that lovastatin distributes rapidly into all tissues and particularly the liver.

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Correspondence to Zhaoyong Yang.

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Zhang, Z., Yang, Z. HPLC Determination of Lovastatin in Rat Tissue. Chroma 66, 487–491 (2007). https://doi.org/10.1365/s10337-007-0395-3

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  • DOI: https://doi.org/10.1365/s10337-007-0395-3

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