Skip to main content
Download PDF

Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells

Molecular Cancer volume 12, Article number: 44 (2013) Cite this article

Abstract

Background

All-trans retinoic acid (ATRA) is currently being used in clinical trials for cancer treatment. The use of ATRA is limited because some cancers, such as lung cancer, show resistance to treatment. However, little is known about the molecular mechanisms that regulate resistance to ATRA treatment. Akt is a kinase that plays a key role in cell survival and cell invasion. Akt is often activated in lung cancer, suggesting its participation in resistance to chemotherapy. In this study, we explored the hypothesis that activation of the Akt pathway promotes resistance to ATRA treatment at the inhibition of cell survival and invasion in lung cancer. We aimed to provide guidelines for the proper use of ATRA in clinical trials and to elucidate basic biological mechanisms of resistance.

Results

We performed experiments using the A549 human lung adenocarcinoma cell line. We found that ATRA treatment promotes PI3k-Akt pathway activation through transcription-independent mechanisms. Interestingly, ATRA treatment induces the translocation of RARα to the plasma membrane, where it colocalizes with Akt. Immunoprecipitation assays showed that ATRA promotes Akt activation mediated by RARα-Akt interaction. Activation of the PI3k-Akt pathway by ATRA promotes invasion through Rac-GTPase, whereas pretreatment with 15e (PI3k inhibitor) or over-expression of the inactive form of Akt blocks ATRA-induced invasion. We also found that treatment with ATRA induces cell survival, which is inhibited by 15e or over-expression of an inactive form of Akt, through a subsequent increase in the levels of the active form of caspase-3. Finally, we showed that over-expression of the active form of Akt significantly decreases expression levels of the tumor suppressors RARβ2 and p53. In contrast, over-expression of the inactive form of Akt restores RARβ2 expression in cells treated with ATRA, indicating that activation of the PI3k-Akt pathway inhibits the expression of ATRA target genes.

Conclusion

Our results demonstrate that rapid activation of Akt blocks transcription-dependent mechanism of ATRA, promotes invasion and cell survival and confers resistance to retinoic acid treatment in lung cancer cells. These findings provide an incentive for the design and clinical testing of treatment regimens that combine ATRA and PI3k inhibitors for lung cancer treatment.

Background

Lung cancer is the leading cause of deaths due to cancer worldwide [1]. Sixty percent of cases are diagnosed in advanced stages, with a life expectancy of less than one year [2]. Chemotherapy treatment is typically administered in these stages; however, the response rate is only about 9% [3]. Clinical trials have shown potential for chemical compounds in cancer treatment such as all-trans retinoic acid (ATRA), which shows anti-proliferative and apoptotic effects and a role in modulating cellular invasion [4]. ATRA exerts its cellular effects by inducing changes in gene expression and is now also thought to be a rapid modulator of signaling pathways involved in cancer. However, the mechanisms mediating these rapid effects are not yet well understood.

ATRA is a biologically active metabolite of vitamin A that regulates diverse cellular functions such as differentiation, proliferation and apoptosis [5–7]. The functions of ATRA are mediated by nuclear receptors, specifically the retinoic acid receptors (RAR α, β, and γ) and the retinoic X receptors (RXR α, β, and γ). RARs act as retinoid-inducible transcriptional factors and can form heterodimers with RXRs, which regulate the expression of genes involved in cell cycle arrest, cell differentiation and cell death [8]. The RARβ2 gene is one of the genes whose expression increases upon ATRA treatment. RARβ2 is a tumor suppressor whose expression is regulated by RARα in response to ATRA [9] and several reports indicate that the expression of RARβ2 is significantly decreased in human cancers [10].

Recent studies have demonstrated that ATRA induces rapid, transcription-independent activation of the PI3k/Akt pathway in neuroblastoma cells [11]. However, the molecular mechanisms by which ATRA promotes activation of the PI3k/Akt pathway are still unknown. The PI3k/Akt pathway is deregulated in most human cancers, including non-small cell lung cancer (NSCLC) [12–14]. Phosphoinositide 3-kinase (PI3k) is activated by stimulation of multiple receptor tyrosine kinases and G protein-coupled receptors. Active PI3k catalyzes the production of phosphatidylinositol-3,4,5-triphosphate (PIP(3)) at the plasma membrane, which in turn promotes the recruitment and activation of Akt at the membrane [15]. Akt is a serine/threonine kinase that plays a key role in multiple cellular processes, such as proliferation, survival and cell invasion [16]. Over-activation of Akt influences multiple downstream effectors, including inactivation of proapoptotic factors such as Bad and caspase-9 [17, 18].

ATRA is currently being used in clinical trials for lung cancer treatment; however, its use is limited because lung cancers show resistance to treatment with ATRA [19–22]. Little is known about the molecular mechanisms that regulate resistance to ATRA treatment in lung cancer. In this report, we tested the hypothesis that Akt mediates resistance to ATRA treatment by treating A549 cells with ATRA and assessed the functional relevance of Akt inactivation in apoptosis and invasion. The A549 cell line is highly invasive, metastatic and resistant to proliferative and survival inhibitory effects of ATRA [23–25].

Results

ATRA promotes activation of the PI3k/Akt pathway by inducing the association of RARα with Akt via transcription-independent mechanisms

To investigate the molecular mechanisms of ATRA resistance in lung cancer cells, we investigated the effects of ATRA in regulating the PI3k/Akt pathway in the ATRA-resistant A549 cell line [26, 27]. The results revealed a rapid activation of the PI3k/Akt pathway, measured by Akt phosphorylation at its serine 473, within 5 min of ATRA treatment and until 60 min after treatment (Figure 1A). Similar results were obtained for H1944, another lung adenocarcinoma cell line, whereas in NL-20, a normal lung cell line, Akt phosphorylation was only detected at 15 min of treatment (Additional file 1: Figure S1). To examine the transcription-dependent action of ATRA on Akt activation, we used BMS493, a pan-retinoic acid receptor antagonist (Figure 1A). Interestingly, treatment with BMS493 did not prevent Akt activation. The effectiveness of BMS493 treatment was evaluated by testing its ability to counteract the transcription-dependent effect of ATRA on p53 expression [28]. As expected, BMS493 inhibited the ATRA-induced increase in p53 expression levels (Figure 1B).

Figure 1
figure 1

ATRA activates the Akt pathway through non-genomic mechanisms in A549 cells .(A) Left, A549 cells were serum-starved for 18 h, treated or non-treated (NT) with 5 μM of ATRA, for the times indicated. Right, A549 cells were preincubated for 1 h with 3 μM of BMS493 before ATRA treatment and total extracts were prepared. The phosphorylated form of Akt and total proteins were detected by western blot using specific antibodies. The bottom graph represents the densitometric values of Akt phosphorylation of three independent experiments (means ± SEM, *P < 0.05; **P < 0.001 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test). (B) A549 cells were serum-starved and treated with ATRA with or without BMS493 for 48 h. Total proteins were detected by western blot.

Full size image

Since ATRA promotes Akt activation, we decided to test whether Akt interacts with components of ATRA signaling. RARα is a major mediator of non-genomic ATRA effects and is widely expressed in all tissue types [29, 30]. To determine whether Akt interacts with RARα, we immunoprecipitated RARα from non-treated or ATRA treated cells. As show in Figure 2A and B, ATRA treatment promoted a significant increase in the interaction between Akt and RARα, with RARα showing a higher binding affinity to the phosphorylated form of Akt. We next determined whether the activation of Akt depends on its interaction with RARα. For this, we tested whether the interaction between RARα and Akt could be competed with APPL1, a protein that interacts directly with Akt [31–33]. Figure 2B shows that over-expression of APPL1 blocks the interaction between RARα with Akt, and inhibits ATRA-mediated Akt activation.

Figure 2
figure 2

ATRA promotes Akt activation mediated by RARα-Akt interaction. (A) RARα was immunoprecipitated from A549 cells treated or non-treated with 5 μM of ATRA for 15 min. Immunoprecipitated RARα and associated protein were detected by western blot. Control refers to immunoprecipitation performed with an Erk1 antibody. The bottom graph shows the results of densitometric analyses of Akt bound to RARα obtained from three independent experiments (means ± SEM, *P < 0.05 compared with non-treated cells (NT) assessed by t test analysis). (B) RARα was immunoprecipitated from A549 cells transfected with EGFP-APPL1 or empty vector and treated with 5 μM of ATRA for 15 min. Association of RARα with Akt was detected by western blot using specific antibodies. Image shows one representative experiment of three independent.

Full size image

ATRA stimulates the translocation of RARα to the plasma membrane, activates Rac and increases membrane ruffles

To determine the influence of ATRA on the subcellular distribution of RARα and Akt, A549 cells were treated with ATRA for different amounts of time and localization of these proteins was examined by immunofluorescence (Figure 3). In non-treated cells, RARα was predominantly found in the nucleus and Akt was located in the plasma membrane and cytoplasm. In contrast, cells treated with ATRA showed RARα recruitment to the plasma membrane from the 5th min to the 15th min of treatment and RARα was co-localized with Akt in newly formed ruffles (white arrows in Figure 3).

Figure 3
figure 3

ATRA promotes recruitment of RARα to the plasma membrane . A549 cells were serum-starved and treated with 5 μM of ATRA for the times indicated. Then cells were fixed and incubated with anti-RARα and anti-Akt followed by incubation with anti-mouse Alexa Fluor 532 and Alexa Fluor 647, respectively, as described in Materials and Methods. Finally, the cells were analyzed by confocal microscopy.

Full size image

Activation of Rac-GTPase is a critical step leading to membrane protrusion and ruffle formation [34, 35]. To assess whether ATRA stimulates Rac activation, we evaluated the interaction of recombinant PAK (p21-activated kinase) with GTP-Rac by pull-down. As shown in Figure 4A, the amount of GTP-bound Rac increased in a time-dependent manner in cells treated with ATRA, whereas the pretreatment of cells for 1 h with PI3k inhibitor (15e) prevented Rac activation.

Figure 4
figure 4

ATRA stimulates Rac activation and promotes invasion. (A) Left, A549 cells were serum-starved for 18 h and treated with 5 μM of ATRA for the times indicated. Other cells were preincubated for 1 h with 5 μM of 15e. Activated Rac was detected with the Rac1 Activation assay kit according to the manufacturer’s instructions. Right, the graph shows the results of densitometric analysis of relative increase of Rac activation obtained in three independent experiments. (B) Cell invasion was analyzed by QCM™ 24–well Invasion Assay Kit. A549 cells were transfected with Myr-Akt, Akt-K179M or empty vector and seeded at 2.5 × 105 cells/well into the upper chamber. DMEM/F12 was added to the lower chamber with or without 5 μM ATRA for 48 h. The invasive cells were detected according to the manufacturer’s instructions. The graphs shows the results of three independent experiments (means ± SEM, *P < 0.05 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test).

Full size image

ATRA promotes cell invasion

The Akt signaling pathway has been previously implicated in cell invasion. To determine the functional consequences of Akt activation by ATRA, we transiently transfected A549 cells with a constitutively active form of Akt (Myr-Akt) and an inactive form of Akt (K179M) and evaluated invasion. As shown in Figure 4B, ATRA promoted invasion in cells expressing empty vector and over-expression of Myr-Akt increased invasion in cells regardless of treatment with ATRA. However, over-expression of Akt-K179M blocked the effect of ATRA on invasion.

Inhibition of the PI3k/Akt pathway blocks the ATRA-dependent survival effect by activating caspase-3

We investigated the effects of ATRA on cell apoptosis by TUNEL assays. As shown in Figure 5A and B, ATRA protected A549 cells against apoptosis under stress conditions, such as ultraviolet (UV) radiation exposition and serum starvation, whereas treatment with PI3k inhibitor (15e) strongly promoted apoptosis (Figure 5B). The combined treatment with ATRA and 15e did not exert additive effects on apoptosis. To investigate the molecular mechanism of PI3k inhibitor-induced apoptosis in A549 cells, the expression of activated caspase-3 was determined by immunofluorescence microscopy. As shown in the bottom panel of Figure 5C, PI3k inhibitor (15e) treatment induced caspase-3 activation, whereas ATRA treatment alone did not affect caspase-3 activation.

Figure 5
figure 5

Inhibition of the PI3k/Akt pathway promotes apoptosis by activation of caspase-3. (A) Left, A549 cells were serum-starved and treated or non-treated (control) with ATRA for 48 h, during the first 12 h after treatment with ATRA, the cells were irradiated with 150 J/m2 of UV-C light for 30 min. Subsequently, DNA fragmentation was detected by TUNEL according to the manufacturer’s instructions. The apoptotic cells are stained brown. Bar, 20 μm. Right, percentages of TUNEL-positive cells were quantified by counting 200 cells from four random microscopic fields (means ± SEM, *P < 0.05 compared with non-treated cells (control) assessed by t test analysis). (B) A549 cells were treated for 48 h with 5 μM of ATRA alone or combined with 5 μM of 15e. Subsequently, DNA fragmentation was detected by TUNEL. Control cells were non-treated. Percentages of TUNEL-positive cells were quantified by counting 200 cells from four random microscopic fields. Means ± SEM, *P < 0.05; **P < 0.001 compared with non-treated cells (control) (analysis of variance and Newman-Keuls test). (C) A549 cells were serum-starved and treated or non-treated (control) with 5 μM of ATRA alone or combined with 5 μM of 15e for 48 h. The cells were fixed, stained with anti-cleaved caspase-3 followed by donkey anti-goat FITC as described in Materials and Methods and analyzed by fluorescence microscopy. Bar, 20 μm.

Full size image

To investigate the direct effect of Akt on apoptosis in cells treated with ATRA, we transfected A549 cells with an active and inactive form of Akt. Figure 6 shows that over-expression of Myr-Akt increase the protective effects of ATRA on apoptosis, whereas over-expression of Akt-K179M promoted apoptosis in cells treated with ATRA. These results demonstrate that PI3k/Akt activation mediates the protective effect of ATRA on apoptosis.

Figure 6
figure 6

Inactive form of Akt (K179M) blocks the ATRA-dependent survival effect. (A) A549 cells were transfected with Myr-Akt, Akt-K179M or empty vector and subsequently treated or non-treated with 5 μM of ATRA for 48 h. Subsequently, DNA fragmentation was detected by TUNEL according to the manufacturer’s instructions. Control cells were non-treated. The apoptotic cells are stained brown. (B) Percentages of TUNEL-positive cells were quantified by counting 100 cells from three random microscopic fields. Means ± SEM, *P < 0.05; **P < 0.001 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test). Bar, 20 μm.

Full size image

Activation of Akt blocks the ATRA-dependent transcription

To determine the effects of Akt on expression of target genes of ATRA such as RARβ2 and p53, we assessed the effect of ATRA in A549 cells transfected with an active and inactive form of Akt. Figure 7A shows that ATRA treatment significantly increased RARβ2 expression in cells transfected with the empty vector, whereas over-expression of Myr-Akt blocked ATRA-induced expression of RARβ2. However, over-expression of Akt-K179M enhanced the effect of ATRA on RARβ2 expression and similar results were obtained in cells treated with PI3k inhibitor (Additional file 2: Figure S2). Figure 7B shows that over-expression of Myr-Akt blocks the expression of p53 in cells treated with ATRA, whereas pretreatment with proteasome inhibitor (MG132) did not prevent Akt-induced decrease in p53 expression. Taken together, these results demonstrate that Akt activation promotes the down-regulation of RARβ2 and p53 at transcriptional level.

Figure 7
figure 7

Akt activation promotes the down-regulation of RARβ2 and p53. (A) Left, A549 cells were transfected with Myr-Akt, Akt-K179M or empty vector and subsequently treated or non-treated with 5 μM of ATRA for 48 h. Total extracts were prepared and levels of protein were detected by western blot. Right, the graph shows the results of densitometric analysis of relative RARβ2 protein expression levels, obtained in three independent experiments (means ± SEM, *P < 0.05 compared with non-treated cells (NT) transfected with empty vector (analysis of variance and Newman-Keuls test). (B) A549 cells were transfected with Myr-Akt and subsequently treated or non-treated with 5 μM of ATRA for 48 h. For the last 24 h of the 48 h treatment period, the cells were incubated with 20 μM of MG132. Total extracts were prepared and levels of protein were detected by western blot using specific antibodies. The image shows one representative experiment of three independent. (C) A549 cells were serum-starved and treated or non-treated (control) with 5 μM of ATRA alone or in combination with 5 μM of 15e for 24 h. The proliferative effect was assessed by BrdU labeling according to the manufacturer’s instructions. The graph shows the results of three independent experiments (means ± SEM, *P < 0.05: **P < 0.001 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test).

Full size image

Combined treatment of ATRA and PI3k inhibitor exerted a modest anti-proliferative effect

To examine the effect of ATRA on cell proliferation, A549 cells were treated for 24 h with ATRA or 15e. As shown in Figure 7C, neither ATRA nor 15e treatment affected proliferation when compared with the control (non-treated cells). Nevertheless, the combination of ATRA with 15e showed a modest anti-proliferative effect. Similar results were obtained when treatment was until 48 and 72 h (data not shown). These results suggest that the PI3k/Akt pathway partially regulates A549 cell proliferation.

Discussion

ATRA is used in clinical trials to suppress the development of different types of cancer [26]. However, its effectiveness is limited in some cancers, such as lung cancer [20, 21, 36]. In this work, we demonstrate that resistance to ATRA-induced apoptosis and suppression of invasion of A549 lung cancer cells is mediated by activation of the PI3k/Akt pathway. Our results show that ATRA promotes phosphorylation of Akt through transcription-independent mechanisms. These data are consistent with reports showing that ATRA induces phosphorylation of Akt via transcription-independent mechanisms in neuroblastoma cells [11]. These results are supported by the use of pan-RAR antagonist (BMS493), which prevent expression of ATRA target genes, but not prevent Akt activation by ATRA. Such results suggest that the structural changes in retinoic acid receptors promoted by BMS493 increase its affinity for co-repressors in the nucleus, whereas in plasma membrane, these structural changes not prevent assembly of Akt-RAR complex. In agreement with this possibility, recent reports indicate that selective receptor modulators can display agonistic or antagonistic function influenced by the subcellular localization [37, 38]. ATRA exerts its transcriptional actions by binding to nuclear receptors. Since Akt activation is independent of transcriptional mechanisms and RARα is the major mediator of transcription-independent ATRA effects [30], we explored the possible association between RARα and Akt. Our results show that RARα interacted with and activated Akt in response to ATRA treatment, which is consistent with the finding that over-expression of RARα increases Akt phosphorylation in COS-7 cells [11]. In addition, RARα is recruited to the plasma membrane, where it became co-localized with Akt in response to ATRA treatment. These results suggest that ATRA promotes the formation of a signaling complex at the plasma membrane in a RARα-dependent manner. Consistent with these data, a pool of RARα is located in lipid rafts forming complexes with signaling proteins as Gαq in response to retinoic acid [39]. RARα has been shown to interact with PI3k at the plasma membrane [11]. The formation of this signaling complex at the plasma membrane regulates Rac activation through the PI3k/Akt pathway to promote cellular invasion, a result that is consistent with the finding that ATRA promotes activation of Rac in neuroblastoma cells [40] and increases the invasion of pancreatic cancer cells [7, 41] and promotes MMP-9 expression through RARα [42]. In addition, we evaluated the effect of ATRA treatment on apoptosis. The results showed that ATRA exerts a protective effect against apoptosis. However, PI3k/Akt pathway inhibition promoted apoptosis via activation of caspase-3. Studies in acute promyelocytic leukemia cells have shown that treatment with the PI3k inhibitor reverses the protective effect of ATRA against apoptosis [43]. Additionally, recent reports have shown that Akt activation suppresses the transactivation of RARα in lung cancer cells [44]. This suggests that Akt negatively modulates the transcriptional actions of ATRA by inhibiting the expression of tumor suppressor genes such as RARβ2 and p53. To address this issue, we evaluated the expression of RARβ2, one of the target genes of ATRA. Our results showed that the over-expression of an active form of Akt (Myr-Akt) blocks the expression of RARβ2, whereas the inactive form of Akt (Akt-K179M) or PI3k inhibitor treatment increases the expression of RARβ2. In addition, over-expression of Myr-Akt substantially reduces p53 expression, other target gene of ATRA [28, 45], whereas treatment with proteasome inhibitor (MG132) not restores p53 expression, indicating that Akt regulates p53 expression to transcriptional level. Consistent with these results, the PI3k/Akt pathway induces the down-regulation of RARβ2 mRNA and protein levels [27, 46]. Finally, we tested the role of the PI3k/Akt pathway in cell proliferation. The results showed that treatment with PI3k inhibitor (15e) exerts a modest anti-proliferative effect. These results indicate that another kinase, such as ERK, regulates proliferation in lung cancer cells.

Taken together, our results suggest that targeting the PI3k-Akt signaling pathway is a potential therapeutic strategy against ATRA-resistance in lung cancer. Follow-up experiments, such as proteomic analyses using mass spectrometry to identify scaffold proteins that regulate the complex assembly of the PI3k-Akt pathway, will be worthwhile for improving our understanding of this proposed mechanism. In agreement with this proposal, recent reports show that cellular retinol–binding protein-I (CRBP-I) decreases the heterodimerization of the catalytic subunit of PI3k with its regulatory subunit in transformed breast cell lines [47]. Based on the results in this study, we propose a model depicting the mechanism of ATRA resistance in lung cancer, as shown in Figure 8. In our model, ATRA binds to RARα to promote its localization at the plasma membrane (step 1). RARα subsequently promotes the recruitment and activation of the PI3k-Akt pathway. The formation of this signaling complex suggests the involvement of scaffold proteins in its assembly (step 2). Akt activation promotes cellular survival and cellular invasion through Rac-GTPase (step 3). Akt suppresses the transactivation of RARα and decreases the expression of RARβ2 (step 4). PI3k-Akt inhibition with 15e or over-expression of an inactive form of Akt (K179M) blocks survival and invasion, restoring the expression of tumor suppressors RARβ2 and p53 (step 5).

Figure 8
figure 8

Model depicting the molecular mechanism of ATRA resistance in lung cancer. ATRA promotes RARα recruitment to the membrane, where it activates the PI3k-Akt pathway (1–2). Akt activation promotes cellular survival and cellular invasion (3). Akt represses RARβ2 and p53 expression (4). PI3k-Akt inhibition restores sensitivity to ATRA treatment and blocks survival and invasion (5).

Full size image

Conclusions

In this study, we provide information on new molecular mechanisms by which lung cancer cells become resistant to ATRA treatment. Our results demonstrate that ATRA promotes PI3k-Akt activation via transcription-independent mechanisms mediated by the RARα-Akt interaction. PI3k-Akt activation by ATRA promotes invasion through Rac-GTPase activation and cell survival, whereas treatment combining ATRA and a PI3k inhibitor or over-expression of an inactive form of Akt suppresses invasion and cell survival, increasing the levels of active caspase-3 and the tumor suppressor RARβ2. In conclusion, activation of Akt blocks the transcriptional effects of ATRA, promotes invasion and cell survival, and confers resistance to retinoic acid treatment in lung cancer cells. These findings provide strategies for the design of drugs that combine ATRA and PI3k inhibitors for lung cancer treatment, a treatment modality that should be clinically evaluated.

Materials and methods

Cell lines and treatments

A549 cells were routinely grown in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS), 100 IU/ml penicillin, 100 μg/ml streptomycin at 37°C in a 5% CO2 atmosphere. All-trans retinoic acid (ATRA) was purchased from Sigma-Aldrich. The PI3k kinase inhibitor, 15e (3-[4-(4-morpholinyl) thieno [3,2-d]pyrimidin-2-yl]-phenol), was purchased from Enzo Life Science and the pan-retinoic acid receptor inverse agonist BMS 493 (4-[(1E)-2-[5,6-dihydro-5,5-dimethyl-8-(2-phenylethynyl)-2-naphthalenyl]ethenyl]benzoic acid), was purchased from Tocris Bioscience. The proteasome inhibitor MG132, was purchased from Sigma-Aldrich. The different compounds were dissolved in dimethylsulfoxide and added to the culture medium at the indicated concentrations.

Western blot and immunoprecipitation

Whole-cell extracts were obtained by lysis of A549 cells in lysis buffer [20 mM Tris–HCl (pH 7.5), 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM sodium vanadate, 1 mM NaF, 10 mM β-glycerophosphate, 1 mM phenylmethylsulfonyl fluoride, and 1.2 mg/ml complete protease inhibitor cocktail; Roche]. The protein extracts were forced through a 22-gauge needle 10 times and centrifuged for 10 min at 14,000 rpm at 4°C and protein concentration was determined by the bicinchoninic acid BCA Protein Assay (Pierce). Approximately 25 μg of protein were separated on 10% SDS-PAGE and transferred to PVDF membranes and then incubated with primary antibodies: anti-phospho-Akt (sc-7985-R; Santa Cruz), anti-Akt (P-2482; Sigma-Aldrich), anti-p53 (sc-126; Santa Cruz) and anti-actin (sc-1616; Santa Cruz). Immunodetection was performed using a fluorescent substrate system (Millipore). Densitometry analysis of western blots was performed using the public domain NIH ImageJ software.

The interactions between endogenous RARα receptors and Akt was assessed in A549 cells that were serum-starved for 18 h and stimulated with 5 μM ATRA, as indicated in the figures. Confluent cultures were washed with PBS, followed by lysis at 4°C. The protein extracts were forced through a 22-gauge needle 10 times and centrifuged for 10 min at 14,000 rpm at 4°C. The supernatants were incubated for 12 h at 4°C with 5 μg/ml anti-RARα (MCA4135Z; Serotec). The immune complexes were recovered by incubation for 2 h at 4°C with protein G-sepharose (25 μl, 10–1241; Invitrogen). Beads were washed three times with lysis buffer and boiled in 1× Laemmli sample buffer. Immunoprecipitated proteins were fractionated on 10% SDS-PAGE and transferred to a PVDF membrane (Millipore). Expression of proteins and putative interactions were detected by western blot using an anti-Akt antibody (P-2482; Sigma-Aldrich). The mouse monoclonal anti-rabbit IgG, light chain specific antibody (211-032-171; Jackson Immuno Research) was used to detect primary antibody.

Immunofluorescence

A549 cells were grown on coverslips precoated with poly-L-lysine and the cells were serum-starved for 18 h and stimulated with 5 μM ATRA for the indicated times. Then, cells were fixed with 4% paraformaldehyde in PBS for 20 min at room temperature, washed three times with PBS, permeabilized with methanol for 6 min at -20°C and blocked with 1% BSA in PBS for 30 min. The cells were then incubated with the primary antibodies. In some experiments, cells were incubated with anti-RARα (MCA4135Z; Serotec) and anti-Akt (P-2482; Sigma-Aldrich) or anti-cleaved caspase-3 (sc-22171; Santa Cruz) followed by incubation with anti-mouse Alexa Fluor 532, anti-mouse Alexa fluor 647 or anti-goat FITC (sc-2024; Santa Cruz), respectively. The cells on coverslips were mounted on glass slides using Vectashield (Vector Laboratories). To visualize the subcellular distribution of RARα and Akt, the images were acquired with a FV1000 confocal laser-scanning microscope (Olympus) using a 63× objective, and for caspase-3 activation, the images were acquired with an Axiovert 40 CFL fluorescence microscope (Carl Zeiss) using a 100× objective.

Rac activation assay

Activation of Rac-GTPase was assessed using the Rac activation assay kit (Millipore) according to the manufacturer’s indications. Briefly, cells were preincubated with 5 μM of 15e for 1 h and stimulated with 5 μM of ATRA, as indicated in the figure legends. Cell lysates were incubated with p21-activated kinase (PAK) binding domain-tagged agarose (10 μg) at 4°C for 2 h. The agarose beads were washed three times with lysis buffer (Millipore) supplemented with phosphatase inhibitors and boiled for 5 min in 1× Laemmli sample buffer. Activated Rac was detected by western blot with Rac antibody (Millipore).

Transfection

For transient transfection, cells were transfected using Lipofectamine™ LTX plus reagent (Invitrogen) according to the manufacturer’s indications. The total amount of DNA in transfections was 4 μg/plate; the assay was performed 48 h after transfection. Expression of transfected constructs was determined by western blot using anti-HA monoclonal antibodies (Covance) and anti-GFP (MMS-118R; Covance). DNA constructs pcDNA3-Myr-HA-Akt, pEGFPC1-human APPL1 and pCMV5-HA-Akt-DN (K179M) were obtained from Addgene, a non-profit plasmid repository (http://www.addgene.org/).

Invasion assay

Cell invasion was carried out using QCM 24-Well Cell Invasion Assay (Millipore) according to the manufacturer’s instructions. Briefly, the extracellular matrix of the insert (8 μm pore size) was rehydrated with serum-free medium, which was subsequently replaced with 250 μl of prepared serum-free suspension of cells transfected with empty vector, Myr-Akt or Akt K179M (1.0 × 106 cells/ml). Then, 500 μl of medium containing 5 μM of ATRA was added to the lower chamber of the insert. Cells were incubated at 37°C in a 5% CO2 atmosphere for 24 h. Finally, cells were dissociated from the membrane according to the manufacturer’s instructions and then detected with CyQuant GR Fluorescent Dye. Fluorescence was measured at 480/520 nm in a Tecan Infinite M1000 plate reader.

TUNEL assay

Detection of apoptosis was performed using the DeadEnd colorimetric TUNEL assay kit (Promega) according to the manufacturer’s instructions. Briefly, A549 cells were grown on coverslips precoated with poly-L-lysine and treated for 48 h with 5 μM of ATRA with or without 5 μM of 15e. After treatment, the cells were fixed with 4% paraformaldehyde in PBS and permeabilized with 0.2% Triton X-100 in PBS. Cells were incubated with recombinant terminal deoxynucleotidyl transferase (rTdT) and biotinylated nucleotides. Endogenous peroxidases were blocked with 0.3% hydrogen peroxide in PBS. The cells were incubated with Streptavidin-HRP, which binds to biotinylated nucleotides incorporated at the 3′-OH DNA ends present in apoptotic cells. Streptavidin-HRP labeled cells were detected by hydrogen peroxide and diaminobenzidine (DAB).

Proliferation assay

A549 cells were seeded in a 96-well plate at a concentration of 10,000 cells/well in 100 μl of DMEM/F12. The cells were treated for 24 h with 5 μM of ATRA with or without 5 μM of 15e. Cell proliferation was measured using the 5-bromo-2′-deoxyuridine (BrdU) enzyme-linked immunosorbent assay (Roche) according to the manufacturer’s instructions. For the last 6 h of the 24 h treatment period, the cells were pulsed with BrdU. Absorbance at 370 and 492 nm was measured in a Tecan Infinite M1000 plate reader.

Statistical analysis

Statistical significances of the differences among data were determined by analysis of variance and Newman-Keuls test or t test, when appropriate, using GraphPad Prism 5.0 software. P < 0.05 was considered as statistically significant. Values are presented as means ± SEM.

Abbreviations

ATRA:

All-trans retinoic acid

RARs:

Retinoic acid receptors

TUNEL:

Terminal deoxynucleotidyl transferase dUTP nick end labeling

NSCLC:

Non-small cell lung cancer.

References

  1. Brzezianska E, Dutkowska A, Antczak A: The significance of epigenetic alterations in lung carcinogenesis. Mol Biol Rep. 2013, 40: 309-325. 10.1007/s11033-012-2063-4

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Kim HSIH, Choi YS, Kim K, Shim YM, Kim J: Surgical resection of recurrent lung cancer in patients following curative resection. J Korean Med Sci. 2006, 21: 224-228. 10.3346/jkms.2006.21.2.224

    Article  PubMed Central  PubMed  Google Scholar 

  3. Hanna N, Shepherd FA, Fossella FV, Pereira JR, De Marinis F, von Pawel J, Gatzemeier U, Tsao TC, Pless M, Muller T, Lim HL, Desch C, Szondy K, Gervais R, Shaharyar Manegold C, Paul S, Paoletti P, Einhorn L, Bunn PA: Randomized phase III trial of pemetrexed versus docetaxel in patients with non-small-cell lung cancer previously treated with chemotherapy. J Clin Oncol. 2004, 22: 1589-1597. 10.1200/JCO.2004.08.163

    Article  CAS  PubMed  Google Scholar 

  4. Freemantle SJ, Spinella MJ, Dmitrovsky E: Retinoids in cancer therapy and chemoprevention: promise meets resistance. Oncogene. 2003, 22: 7305-7315. 10.1038/sj.onc.1206936

    Article  CAS  PubMed  Google Scholar 

  5. Nowak D, Stewart D, Koeffler HP: Differentiation therapy of leukemia: 3 decades of development. Blood. 2009, 113: 3655-3665. 10.1182/blood-2009-01-198911

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Hormi-Carver K, Feagins LA, Spechler SJ, Souza RF: All trans-retinoic acid induces apoptosis via p38 and caspase pathways in metaplastic Barrett’s cells. Am J Physiol Gastrointest Liver Physiol. 2007, 292: G18-G27.

    Article  CAS  PubMed  Google Scholar 

  7. Leelawat K, Ohuchida K, Mizumoto K, Mahidol C, Tanaka M: All-trans retinoic acid inhibits the cell proliferation but enhances the cell invasion through up-regulation of c-met in pancreatic cancer cells. Cancer Lett. 2005, 224: 303-310. 10.1016/j.canlet.2004.10.016

    Article  CAS  PubMed  Google Scholar 

  8. Tang XH, Gudas LJ: Retinoids, retinoic acid receptors, and cancer. Annu Rev Pathol. 2011, 6: 345-364. 10.1146/annurev-pathol-011110-130303

    Article  CAS  PubMed  Google Scholar 

  9. Pozzi S, Rossetti S, Bistulfi G, Sacchi N: RAR-mediated epigenetic control of the cytochrome P450 Cyp26a1 in embryocarcinoma cells. Oncogene. 2006, 25: 1400-1407. 10.1038/sj.onc.1209173

    Article  CAS  PubMed  Google Scholar 

  10. Xu XC: Tumor-suppressive activity of retinoic acid receptor-beta in cancer. Cancer Lett. 2007, 253: 14-24. 10.1016/j.canlet.2006.11.019

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Masia S, Alvarez S, de Lera AR, Barettino D: Rapid, nongenomic actions of retinoic acid on phosphatidylinositol-3-kinase signaling pathway mediated by the retinoic acid receptor. Mol Endocrinol. 2007, 21: 2391-2402. 10.1210/me.2007-0062

    Article  CAS  PubMed  Google Scholar 

  12. Brognard J, Clark AS, Ni Y, Dennis PA: Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res. 2001, 61: 3986-3997.

    CAS  PubMed  Google Scholar 

  13. Garcia-Echeverria C, Sellers WR: Drug discovery approaches targeting the PI3K/Akt pathway in cancer. Oncogene. 2008, 27: 5511-5526. 10.1038/onc.2008.246

    Article  CAS  PubMed  Google Scholar 

  14. Guo Y, Du J, Kwiatkowski DJ: Molecular dissection of AKT activation in lung cancer cell lines. Mol Cancer Res. 2013, 11: 282-293. 10.1158/1541-7786.MCR-12-0558

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Hennessy BT, Smith DL, Ram PT, Lu Y, Mills GB: Exploiting the PI3K/AKT pathway for cancer drug discovery. Nat Rev Drug Discov. 2005, 4: 988-1004. 10.1038/nrd1902

    Article  CAS  PubMed  Google Scholar 

  16. Garcia-Regalado A, Guzman-Hernandez ML, Ramirez-Rangel I, Robles-Molina E, Balla T, Vazquez-Prado J, Reyes-Cruz G: G protein-coupled receptor-promoted trafficking of Gbeta1gamma2 leads to AKT activation at endosomes via a mechanism mediated by Gbeta1gamma2-Rab11a interaction. Mol Biol Cell. 2008, 19: 4188-4200. 10.1091/mbc.E07-10-1089

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Song G, Ouyang G, Bao S: The activation of Akt/PKB signaling pathway and cell survival. J Cell Mol Med. 2005, 9: 59-71. 10.1111/j.1582-4934.2005.tb00337.x

    Article  CAS  PubMed  Google Scholar 

  18. Hers I, Vincent EE, Tavare JM: Akt signalling in health and disease. Cell Signal. 2011, 23: 1515-1527. 10.1016/j.cellsig.2011.05.004

    Article  CAS  PubMed  Google Scholar 

  19. Fritz H, Kennedy D, Fergusson D, Fernandes R, Doucette S, Cooley K, Seely A, Sagar S, Wong R, Seely D: Vitamin A and retinoid derivatives for lung cancer: a systematic review and meta analysis. PLoS One. 2011, 6: e21107- 10.1371/journal.pone.0021107

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Geradts J, Chen JY, Russell EK, Yankaskas JR, Nieves L, Minna JD: Human lung cancer cell lines exhibit resistance to retinoic acid treatment. Cell Growth Differ. 1993, 4: 799-809.

    CAS  PubMed  Google Scholar 

  21. Petty WJ, Li N, Biddle A, Bounds R, Nitkin C, Ma Y, Dragnev KH, Freemantle SJ, Dmitrovsky E: A novel retinoic acid receptor beta isoform and retinoid resistance in lung carcinogenesis. J Natl Cancer Inst. 2005, 97: 1645-1651. 10.1093/jnci/dji371

    Article  CAS  PubMed  Google Scholar 

  22. Somenzi G, Sala G, Rossetti S, Ren M, Ghidoni R, Sacchi N: Disruption of retinoic acid receptor alpha reveals the growth promoter face of retinoic acid. PLoS One. 2007, 2: e836- 10.1371/journal.pone.0000836

    Article  PubMed Central  PubMed  Google Scholar 

  23. Sussan TE, Pletcher MT, Murakami Y, Reeves RH: Tumor suppressor in lung cancer 1 (TSLC1) alters tumorigenic growth properties and gene expression. Mol Cancer. 2005, 4: 28- 10.1186/1476-4598-4-28

    Article  PubMed Central  PubMed  Google Scholar 

  24. Vinodhkumar R, Song YS, Ravikumar V, Ramakrishnan G, Devaki T: Depsipeptide a histone deacetlyase inhibitor down regulates levels of matrix metalloproteinases 2 and 9 mRNA and protein expressions in lung cancer cells (A549). Chem Biol Interact. 2007, 165: 220-229. 10.1016/j.cbi.2006.12.012

    Article  CAS  PubMed  Google Scholar 

  25. Chang CC, Shih JY, Jeng YM, Su JL, Lin BZ, Chen ST, Chau YP, Yang PC, Kuo ML: Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis. J Natl Cancer Inst. 2004, 96: 364-375. 10.1093/jnci/djh059

    Article  CAS  PubMed  Google Scholar 

  26. Nakajoh M, Fukushima T, Suzuki T, Yamaya M, Nakayama K, Sekizawa K, Sasaki H: Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines. Am J Respir Cell Mol Biol. 2003, 28: 296-304. 10.1165/rcmb.4845

    Article  CAS  PubMed  Google Scholar 

  27. Kawakami S, Suzuki S, Yamashita F, Hashida M: Induction of apoptosis in A549 human lung cancer cells by all-trans retinoic acid incorporated in DOTAP/cholesterol liposomes. J Control Release. 2006, 110: 514-521. 10.1016/j.jconrel.2005.10.030

    Article  CAS  PubMed  Google Scholar 

  28. Zhang H, Rosdahl I: Expression profiles of p53, p21, bax and bcl-2 proteins in all-trans-retinoic acid treated primary and metastatic melanoma cells. Int J Oncol. 2004, 25: 303-308.

    CAS  PubMed  Google Scholar 

  29. Wan YJ, Wang L, Wu TC: Detection of retinoic acid receptor mRNA in rat tissues by reverse transcriptase-polymerase chain reaction. J Mol Endocrinol. 1992, 9: 291-294. 10.1677/jme.0.0090291

    Article  CAS  PubMed  Google Scholar 

  30. Chen N, Napoli JL: All-trans-retinoic acid stimulates translation and induces spine formation in hippocampal neurons through a membrane-associated RARalpha. FASEB J. 2008, 22: 236-245.

    Article  CAS  PubMed  Google Scholar 

  31. Tan Y, You H, Wu C, Altomare DA, Testa JR: Appl1 is dispensable for mouse development, and loss of Appl1 has growth factor-selective effects on Akt signaling in murine embryonic fibroblasts. J Biol Chem. 2010, 285: 6377-6389. 10.1074/jbc.M109.068452

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Saito T, Jones CC, Huang S, Czech MP, Pilch PF: The interaction of Akt with APPL1 is required for insulin-stimulated Glut4 translocation. J Biol Chem. 2007, 282: 32280-32287. 10.1074/jbc.M704150200

    Article  CAS  PubMed  Google Scholar 

  33. Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR: Identification of a chromosome 3p14.3–21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2. Oncogene. 1999, 18: 4891-4898. 10.1038/sj.onc.1203080

    Article  CAS  PubMed  Google Scholar 

  34. Hernandez-Cuevas NA, Hernandez-Rivas R, Sosa-Peinado A, Rojo-Dominguez A, Vargas M: Identification and evaluation of inhibitors of the EhGEF1 protein from Entamoeba histolytica. J Mol Recognit. 2011, 24: 935-944. 10.1002/jmr.1137

    Article  CAS  PubMed  Google Scholar 

  35. Ridley AJ, Paterson HF, Johnston CL, Diekmann D, Hall A: The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell. 1992, 70: 401-410. 10.1016/0092-8674(92)90164-8

    Article  CAS  PubMed  Google Scholar 

  36. Arrieta O, Gonzalez-De la Rosa CH, Arechaga-Ocampo E, Villanueva-Rodriguez G, Ceron-Lizarraga TL, Martinez-Barrera L, Vazquez-Manriquez ME, Rios-Trejo MA, Alvarez-Avitia MA, Hernandez-Pedro N, Rojas-Marin C, De la Garza J: Randomized phase II trial of All-trans-retinoic acid with chemotherapy based on paclitaxel and cisplatin as first-line treatment in patients with advanced non-small-cell lung cancer. J Clin Oncol. 2010, 28: 3463-3471. 10.1200/JCO.2009.26.6452

    Article  CAS  PubMed  Google Scholar 

  37. Smith CL, O’Malley BW: Coregulator function: a key to understanding tissue specificity of selective receptor modulators. Endocr Rev. 2004, 25: 45-71. 10.1210/er.2003-0023

    Article  CAS  PubMed  Google Scholar 

  38. Robinson SP, Jordan VC: Reversal of the antitumor effects of tamoxifen by progesterone in the 7, 12-dimethylbenzanthracene-induced rat mammary carcinoma model. Cancer Res. 1987, 47: 5386-5390.

    CAS  PubMed  Google Scholar 

  39. Piskunov A, Rochette-Egly C: A retinoic acid receptor RARalpha pool present in membrane lipid rafts forms complexes with G protein alphaQ to activate p38MAPK. Oncogene. 2012, 31: 3333-3345. 10.1038/onc.2011.499

    Article  CAS  PubMed  Google Scholar 

  40. Pan J, Kao YL, Joshi S, Jeetendran S, Dipette D, Singh US: Activation of Rac1 by phosphatidylinositol 3-kinase in vivo: role in activation of mitogen-activated protein kinase (MAPK) pathways and retinoic acid-induced neuronal differentiation of SH-SY5Y cells. J Neurochem. 2005, 93: 571-583. 10.1111/j.1471-4159.2005.03106.x

    Article  CAS  PubMed  Google Scholar 

  41. Kunigal S, Ponnusamy MP, Momi N, Batra SK, Chellappan SP: Nicotine, IFN-gamma and retinoic acid mediated induction of MUC4 in pancreatic cancer requires E2F1 and STAT-1 transcription factors and utilize different signaling cascades. Mol Cancer. 2012, 11: 24-

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  42. Zaragoza R, Gimeno A, Miralles VJ, Garcia-Trevijano ER, Carmena R, Garcia C, Mata M, Puertes IR, Torres L, Vina JR: Retinoids induce MMP-9 expression through RARalpha during mammary gland remodeling. Am J Physiol Endocrinol Metab. 2007, 292: E1140-E1148.

    Article  CAS  PubMed  Google Scholar 

  43. Billottet C, Banerjee L, Vanhaesebroeck B, Khwaja A: Inhibition of class I phosphoinositide 3-kinase activity impairs proliferation and triggers apoptosis in acute promyelocytic leukemia without affecting atra-induced differentiation. Cancer Res. 2009, 69: 1027-1036.

    Article  CAS  PubMed  Google Scholar 

  44. Srinivas H, Xia D, Moore NL, Uray IP, Kim H, Ma L, Weigel NL, Brown PH, Kurie JM: Akt phosphorylates and suppresses the transactivation of retinoic acid receptor alpha. Biochem J. 2006, 395: 653-662. 10.1042/BJ20051794

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  45. Mrass P, Rendl M, Mildner M, Gruber F, Lengauer B, Ballaun C, Eckhart L, Tschachler E: Retinoic acid increases the expression of p53 and proapoptotic caspases and sensitizes keratinocytes to apoptosis: a possible explanation for tumor preventive action of retinoids. Cancer Res. 2004, 64: 6542-6548. 10.1158/0008-5472.CAN-04-1129

    Article  CAS  PubMed  Google Scholar 

  46. Lefebvre B, Brand C, Flajollet S, Lefebvre P: Down-regulation of the tumor suppressor gene retinoic acid receptor beta2 through the phosphoinositide 3-kinase/Akt signaling pathway. Mol Endocrinol. 2006, 20: 2109-2121. 10.1210/me.2005-0321

    Article  CAS  PubMed  Google Scholar 

  47. Farias EF, Marzan C, Mira-y-Lopez R: Cellular retinol-binding protein-I inhibits PI3K/Akt signaling through a retinoic acid receptor-dependent mechanism that regulates p85-p110 heterodimerization. Oncogene. 2005, 24: 1598-1606. 10.1038/sj.onc.1208347

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was partly supported by the National Council of Science and Technology of Mexico (CONACYT 181534, 105532 and 115591).

Author information

Authors and Affiliations

  1. Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana, Unidad Cuajimalpa. Artificios 40, Col. Hidalgo, México, D. F, 01120, Mexico

    Alejandro García-Regalado & Claudia Haydée González-De la Rosa

  2. Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, México, D. F, 14740, Mexico

    Miguel Vargas

  3. Subdirección de Investigación Básica, Instituto Nacional de Cancerología, San Fernando 22, Col Sección XVI, México, D. F, 14080, Mexico

    Alejandro García-Carrancá & Elena Aréchaga-Ocampo

Authors
  1. Alejandro García-Regalado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Miguel Vargas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alejandro García-Carrancá
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elena Aréchaga-Ocampo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Claudia Haydée González-De la Rosa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Claudia Haydée González-De la Rosa.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

Conception and design: C H G-D R, A G-R. Financial support: C H G-D R. Collection and assembly of data: A G-R. Data analysis and interpretation: C H G-D R, A G-R. Manuscript writing: A G-R, M V, A G-C, E A-O, C H G-D R. Final approval of manuscript: A G-R, M V, A G-C, E A-O, C H G-D R. All authors read and approved the final manuscript.

Electronic supplementary material

12943_2013_1112_MOESM1_ESM.pdf

Additional file 1: Figure S1: ATRA activates the Akt pathway in H1944 and NL-20 cells. (A) Left, H1944 cells were serum-starved for 18 h and treated or non-treated (NT) with 5 μM of ATRA for the times indicated. Right, NL20 cells were serum-starved for 18 h and treated or non-treated (NT) with 5 μM of ATRA for the times indicated and total extracts were prepared. The phosphorylated form of Akt and total proteins levels were detected by western blot using specific antibodies. (PDF 366 KB)

12943_2013_1112_MOESM2_ESM.pdf

Additional file 2: Figure S2: Inhibition of the PI3k/Akt pathway increased RARβ2 expression. A549 cells were serum-starved for 18 h and preincubated for 1 h with 5 μM of 15e before ATRA treatment. The cells were subsequently treated or non-treated with 5 μM of ATRA for 48 h, total extracts were prepared and levels of protein were detected by western blot. (PDF 121 KB)

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

García-Regalado, A., Vargas, M., García-Carrancá, A. et al. Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells. Mol Cancer 12, 44 (2013). https://doi.org/10.1186/1476-4598-12-44

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1476-4598-12-44

Keywords

Molecular Cancer

ISSN: 1476-4598