Original CommunicationsNicotine induces platelet-derived growth factor release and cytoskeletal alteration in aortic smooth muscle cells☆
Section snippets
Cell culture
Smooth muscle cells were obtained from bovine thoracic aortas by collagenase digestion.22 The cells were seeded into 25 cm2 flasks (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ) in a medium consisting of Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal calf serum (FCS) and antibiotics (penicillin 100 IU/mL, streptomycin 100 μg/mL, and gentamycin 200 μg/mL). The cultures were kept at 37°C in an atmosphere of 5% CO2 in air. The medium was changed after 1 day and
PDGF assay in the conditioned media
SMCs stimulated with (-)-nicotine released more PDGF than the control (P <.05; Table).(-)-Nicotine concentrations PDGF 0 0.43 ± 0.07 6 × 10-8 mol/L 1.00 ± 0.14 6 × 10-7 mol/L 1.24 ± 0.14 6 × 10-6 mol/L 1.00 ± 0.12 6 × 10-5 mol/L 0.87 ± 0.10 6 × 10-4 mol/L 0.61 ± 0.07
Discussion
Epidemiological studies have established a strong correlation between cigarette smoking and the development and progression of atherosclerosis, myocardial infarction, vascular graft failures, and death from coronary artery disease. Smokers have a 2.5-fold increase in the incidence of coronary heart disease, a 1.5-fold increase in stroke mortality, and an even greater increase in peripheral arterial occlusive disease as compared with nonsmokers.24 However, there is a paucity of information
References (36)
- et al.
Nicotine and its metabolite cotinine are mitogenic for human vascular smooth muscle cells
J Vasc Surg
(1997) - et al.
Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth muscle cells
J Vasc Surg
(1996) - et al.
The biology of platelet-derived growth factor
Cell
(1986) - et al.
Identification and structural analysis of the A type receptor for platelet-derived growth factor. Similarities with the B type receptor
J Biol Chem
(1989) Platelet-derived growth factor receptor signals
J Biol Chem
(1994)- et al.
Phosphorylation of phospholipase C*1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton
Exp Cell Res
(1998) Cytokinesis in animal cells
Curr Opin Cell Biol
(1989)- et al.
Mice lacking vimentin develop and reproduce without an obvious phenotype
Cell
(1994) - et al.
Intermediate filaments and cytoplasmic networking: new connections and more functions
Curr Opin Cell Biol
(1997) - et al.
Smooth muscle cells and the pathogenesis of the lesion of atherosclerosis
Br Heart J
(1993)
Cigarette smoking and the development and progression of aortic atherosclerosis: a 9-year population-base follow-up study in women
Circulation
The pathogenesis of atherosclerosis: a perspective for the 1990s
Nature
Atherosclerosis. An inflammatory disease
N Engl J Med
Platelet-derived growth factor (PDGF)-induced actin rearrangement is deregulated in cells expressing a mutant Y778F PDGF β-receptor
J Cell Sci
PDGF induces reorganization of vimentin filaments
J Cell Sci
Regulation of a calcium-dependent tyrosine kinase in vascular smooth muscle cells by angiotensin II and platelet-derived growth factor. Dependence on calcium and the actin cytoskeleton
J Biol Chem
Microfilament structure and function in the cortical cytoskeleton
Annu Rev Cell Biol
Intermediate filaments: regulation of gene expression and assembly
Eur J Biochem
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