Basic and clinical immunologyEosinophilic apoptosis in sinus mucosa: Relationship to tissue eosinophilia and its resolution in allergic sinusitis☆,☆☆
Section snippets
Patients and tissue samples
The 29 patients involved in this study ranged in age from 17 to 69 years. There were 19 men and 10 women. All patients had ethmoid and maxillary sinusitis and had undergone routine diagnostic procedures at the Department of Otolaryngology, Osaka Medical College. Specific IgE for 9 allergens (house dust mites, the major 5 Japanese pollens, dogs, cats, and fungus) was measured by using the CAP-RAST test in all patients. The diagnosis of AS was confirmed by the elevation of total serum IgE and
Eosinophil accumulation in AS
To assess eosinophil accumulation, anti-EG2 antibodies, which recognizes eosinophils in tissue, were used (Fig 1).
This anti-EG2 antibody bound specifically to eosinophil cationic protein secreted (or extracted) from eosinophils. Results are shown in Table I.
Disease Superficial, mean ± SD (median) Deep, mean ± SD (median) P value* AS (n = 13) 114 ± 58 (102)†
Discussion
In this study we revealed that EG2+ cells were abundant in allergic mucosa and dominated the superficial area, pointing to eosinophils as the main effector mediating inflammation in allergic tissue. To explore whether this accumulation was associated with eosinophilic apoptosis, we examined the eosinophil AI in superficial and deep layers of the mucosa. The low AI in the superficial layer was consistent with the theory that inhibition of apoptosis contributes to eosinophil accumulation.10, 11
Acknowledgements
We thank the staff of the Department of Otolaryngology, Osaka Medical College, for excellent assistance with this work.
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Supported in part by grants-in-aid from the Society for Promotion of International Otorhinolaryngology, Japan.
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Reprint requests: Hiroshi Takenaka, MD, Department of Otorhinolaryngology, Osaka Medical College, Daigaku-cho 2-7, Takatsuki, Osaka 0569-8686, Japan.