Neuroprotective Effects in Gerbils of Spiramine T from Spiraea japonica var. acuta

 

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Abstract

The neuroprotective effects of spiramine T, an atisine-type diterpenoid alkaloid isolated from the Chinese herbal medicine Spiraea japonica var. acuta (Rosaceaee), on cerebral ischemia-reperfusion injury produced by 10-min bilateral occlusion of the common carotid arteries followed by 5-day reperfusion in gerbils were investigated. Intravenous spiramine T (0.38, 0.75, and 1.5 mg · kg^-1) markedly reduced the stroke index, enhanced the recovery of EEG amplitude during reperfusion and decreased the concentrations of cortex calcium and LPO in a dose-dependent manner. However, no significant effects on water and sodium contents were observed. These results suggested that spiramine T exhibited protective effects on cerebral ischemia-reperfusion injury in gerbils, and its mechanism might be related to reducing calcium accumulation and lipid peroxidation. This is the first report on spiramine T as a natural product with neuroprotective effects.

Abbreviations

AA:arachidonic acid

EEG:electroencephalogram

LPO:lipid peroxide

MDA:malondialdehyde

PAF:platelet activating factor

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Xiao-Jiang Hao

Kunming Institute of Botany

Chinese Academy of Sciences

Heilongtan

Kunming 650204

Yunnan

China

Email: xjhao@mail.kib.ac.cn

Phone: Tel: +86-871-5150227