Basic-alimentary tractSerotonin Excites Neurons in the Human Submucous Plexus via 5-HT3 Receptors
Section snippets
Tissue Samples
Human tissue samples of small and large bowel were obtained from 28 patients undergoing surgery in the Departments of Surgery at the Medical Clinic in Freising and the Medical Clinic of the Technische Universität in München. Diagnoses that led to the surgery were as follows: carcinoma of the small or large bowel (19 patients), polyposis (1 patient), diverticulitis (3 patients), Crohn’s diseae (1 patient), ovarian cancer (2 patients), pancreatic carcinoma (1 patient), and ulcerative colitis (1
Neuroimaging
Experiments were performed on 24 preparations from 21 patients (10 men, 11 women; mean age: 62.3 ± 15.7 years; range, 31–89 years). A total of 50 ganglia containing 421 nerve cells were analyzed. The mean number of nerve cells per ganglion was 8.4 ± 6.1 (range, 1–40).
Application of 5-HT or the selective 5-HT3 receptor agonist 2-methyl-5-HT to ganglion cells in the submucous plexus resulted in an excitatory response in 33 of 50 ganglia (Figure 1). Only in 2 preparations from 2 patients (4
Discussion
With this study we showed the presence of functional 5-HT3 receptors that on activation excited neurons in the human submucous plexus. This conclusion is based on the finding that the 5-HT3 receptor agonist, 2-methyl-5-HT, mimicked the excitatory 5-HT response, and that a selective concentration of the 5-HT3 antagonist, tropisetron, antagonized the response to 5-HT. The tropisetron-insensitive component of the 5-HT response that was detected in a few enteric nerve cells suggests the involvement
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Supported by DFG Sche 267/5-1, 5-2, and the Wellcome Trust.