Abstract
Transforming growth factor-beta (TGF-β) plays a dual role in hepatocytes, inducing both pro- and anti-apoptotic responses, whose balance decides cell fate. Survival signals are mediated by the epidermal growth factor receptor (EGFR) pathway, which is activated by TGF-β in these cells. Caveolin-1 (Cav1) is a structural protein of caveolae linked to TGF-β receptors trafficking and signaling. Previous results have indicated that in hepatocytes, Cav1 is required for TGF-β-induced anti-apoptotic signals, but the molecular mechanism is not fully understood yet. In this work, we show that immortalized Cav1−/− hepatocytes were more sensitive to the pro-apoptotic effects induced by TGF-β, showing a higher activation of caspase-3, higher decrease in cell viability and prolonged increase through time of intracellular reactive oxygen species (ROS). These results were coincident with attenuation of TGF-β-induced survival signals in Cav1−/− hepatocytes, such as AKT and ERK1/2 phosphorylation and NFκ-B activation. Transactivation of the EGFR pathway by TGF-β was impaired in Cav1−/− hepatocytes, which correlated with lack of activation of TACE/ADAM17, the metalloprotease responsible for the shedding of EGFR ligands. Reconstitution of Cav1 in Cav1−/− hepatocytes rescued wild-type phenotype features, both in terms of EGFR transactivation and TACE/ADAM17 activation. TACE/ADAM17 was localized in detergent-resistant membrane (DRM) fractions in Cav1+/+ cells, which was not the case in Cav1−/− cells. Disorganization of lipid rafts after treatment with cholesterol-binding agents caused loss of TACE/ADAM17 activation after TGF-β treatment. In conclusion, in hepatocytes, Cav1 is required for TGF-β-mediated activation of the metalloprotease TACE/ADAM17 that is responsible for shedding of EGFR ligands and activation of the EGFR pathway, which counteracts the TGF-β pro-apoptotic effects. Therefore, Cav1 contributes to the pro-tumorigenic effects of TGF-β in liver cancer cells.
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Main
The transforming growth factor-beta (TGF-β) belongs to a family of polypeptide factors, whose cytostatic and apoptotic functions help restrain the growth of mammalian cells. However, paradoxically, TGF-β also modulates processes such as cell invasion, immune regulation and microenvironment modification, which cancer cells may exploit to their advantage.1 Indeed, a better knowledge about the mechanistic basis and clinical relevance of TGF-β is required for a better understanding of the complexity and therapeutic potential of this pathway. In hepatocytes, TGF-β induces both pro- and anti-apoptotic signals, whose balance decides cell fate.2 Those hepatocytes that survive to TGF-β-mediated apoptosis induce an epithelial–mesenchymal transition (EMT) process, which confers migratory properties and apoptosis resistance.3 The anti-apoptotic signals are at least partially mediated by the epidermal growth factor receptor (EGFR) pathway, which is transactivated by TGF-β through a mechanism that involves upregulation of the EGFR ligands and activation of the metalloprotease TACE/ADAM17 responsible for their shedding.4, 5
Many efforts have been done in the recent years for a better understanding of spatial requirements on TGF-β signaling, including endocytic TGF-β receptors trafficking. Strong pieces of evidence support that TGF-β receptors can be located both in clathrin-coated pits and caveolin/cholesterol-enriched lipid rafts.6, 7 A pioneer study from Di Guglielmo et al.8 proposed that Smad2 phosphorylation would require clathrin-dependent endocytosis, whereas TGF-β receptors internalization via caveolae/lipid rafts would inhibit its signaling. From then, different studies have suggested that the endocytic pathways’ role on TGF-β signaling depend on the cell type and a general rule about the role of endocytosis in TGF-β signaling is not well understood yet.9 In hepatocytes, Smad activation is in a large extent accomplished on the hepatocyte plasma membrane in an AP-2 complex-dependent manner, being unnecessary the formation of clathrin-coated pits.10 In contrast, the non-Smad/AKT pathway activation requires caveolin-1 (Cav1)-dependent endocytosis,10 which is required for counteracting apoptosis.11
Cav1 is required for caveolae formation, which regulates not only endocytosis, but also lipid metabolism and energy homeostasis.12 The localization of membrane receptors in the lipid raft pushed to investigate the role of Cav1 in regulating signaling events. In the case of epidermal growth factor (EGF) signaling, it was proposed that non-caveolae-coated pits are involved in the compartmentalization and internalization of the EGFR, although caveolin-rich domains may be required for signaling.13 In this line of evidence, different studies revealed an important role for Cav1 in EGFR-induced effects on cell proliferation and migration.14, 15
In this work, we have examined the role of Cav1 in the anti-apoptotic signals induced by TGF-β in hepatocytes, postulating that it may be required for TGF-β-mediated regulation of EGFR signaling. Using different experimental approaches and an immortalized hepatocyte cell line derived from Cav1−/− mice, we demonstrate that transactivation of the EGFR pathway by TGF-β is impaired in Cav1−/− hepatocytes. However, Cav1 is not required for the cellular response to EGFR ligands, but is necessary for TGF-β-mediated activation of the metalloprotease TACE/ADAM17, which is responsible for shedding of EGFR ligands and requires an intact lipid raft domain to be activated by TGF-β.
Results
Cav1 deficiency alters the balance between the pro- and anti-apoptotic signals induced by TGF-β in hepatocytes
In order to analyze the specific relevance of Cav1 in the response to TGF-β, we have used an immortalized neonatal hepatocyte cell line obtained from 3-day old Cav1−/− animals16 (see Materials and Methods section and Supplementary Figure 1a). Cav1−/− hepatocytes showed an increased sensitivity to TGF-β in terms of cell death, exhibiting a significant higher percentage of non-viable cells when compared with Cav1+/+ cells after 72 h of treatment (Figures 1a–c). Activation of caspase-3, as a hallmark of apoptosis, showed similar dynamics, being increased in Cav1-deficient hepatocytes when compared with control cells and remained high after 72 h of TGF-β treatment (Figure 1d). Since TGF-β-induced apoptosis in hepatocytes requires reactive oxygen species (ROS) production mediated by a NADPH oxidase system (NOX4),17 we next analyzed intracellular ROS content. Results indicated that the increase in intracellular ROS content was a transient process in Cav1+/+ hepatocytes showing a maximum increase after 6-h treatment, decreasing at later times up to levels even lower than those found in the untreated cells. However, in knockout cells (Cav1−/−), increase in ROS levels were constantly high through all the time (Figure 1e). All these results indicate that apoptotic features induced by TGF-β in immortalized neonatal hepatocytes are increased in the absence of Cav1 expression. In fetal and neonatal hepatocytes, TGF-β simultaneously induces both pro- and anti-apoptotic signals, whose final balance determines the cell fate.2, 4 Anti-apoptotic signals include the phospho-inositol-3-kinase (PI-3K)/AKT pathway, MAPK/ERKs and NFκ-B pathways. Thus, we next examined whether the increased apoptosis observed in Cav1-deficient hepatocytes is caused by impairment in activation of survival signals, triggered by TGF-β. Western blot analysis showed a significant inhibition of AKT activation and decreased phosphorylation of MAP kinases ERK1/2 in TGF-β-treated Cav1−/− cells (Figures 2a and b). In addition, TGF-β-induced activation of pro-survival NFκ-B signaling was also attenuated in Cav1−/− cells, which showed lower translocation of cytosolic p65 to the nucleus after treatment with the cytokine (Figures 2c and d). Therefore, in the absence of Cav1, the imbalance between pro- and anti-apoptotic regulatory signals finally pushes cells to die.
Cav1 deficiency prevents transactivation of the EGFR pathway by TGF-β in hepatocytes
TGF-β-induced survival signals in hepatocytes and liver tumor cells require transactivation of the EGFR pathway.4, 18 We hypothesized that EGFR transactivation by TGF-β could be perturbed in the absence of Cav1. In Cav1+/+ cells, TGF-β induced phosphorylation of EGFR, examined both by western blot (Figure 3a) and immunocytochemistry (Figure 3b). Inhibition of the EGFR catalytic activity by the tyrphostin AG1478 showed that EGFR-mediated signaling is necessary to rescue Cav1+/+ hepatocytes from the expected TGF-β-induced apoptotic fate (Supplementary Figure 2). However, Cav1−/− cells did not show EGFR phosphorylation in response to TGF-β (Figures 3a and b), and treatment with AG1478 did not alter cell viability (Supplementary Figure 2). Lack of response was not due to differences in EGFR expression, which was similar in Cav1+/+ and Cav−/− cells (Supplementary Figures 1b and c).
To exclude that the absence of EGFR transactivation by TGF-β could be due to a defect acquired during development of the Cav1 knockout mice and not to the mere absence of Cav1, we reconstituted the expression of Cav1 in Cav1−/− cells. Ectopic expression of Cav1 in the immortalized Cav1−/− cell line led to a recovery of the wild-type phenotype, with a decreased sensitivity in terms of cell death (Figure 4a) and lower activation of caspase-3 (Figure 4b). It is important to point out that the control cell line (+IRES-GFP, a control plasmid expressing only GFP) behaved as the parental cell line. Then, activation of AKT and ERK1/2 were examined, evidencing a recovery of AKT phosphorylation and an increase in ERK1/2 phosphorylation in TGF-β-treated Cav1−/− when expression of Cav1 was restored (Figure 4c). Interestingly, restitution of Cav1 rescued the transactivation of EGFR by TGF-β in Cav1−/− hepatocytes (Figure 4d). In order to confirm the results observed in Cav1−/− hepatocytes, we knocked down Cav1 in Cav1+/+ hepatocytes using a specific siRNA approach. Downregulation of Cav1 was efficient using two siRNAs (Supplementary Figure 3) and the EGFR phosphorylation after stimulation with TGF-β was severely reduced in Cav1-silenced Cav1+/+ cells (Supplementary Figure 3). Therefore, we conclude that Cav1 is determinant for the capacity of TGF-β to transactivate the EGFR pathway in hepatocytes.
As we observed that the EGFR transactivation was impaired in Cav1−/− hepatocytes, a possibility was that Cav1 is required for cell responses to extracellular EGFR ligands. Although we realized that the use of heparin-binding EGF-like growth factor (HB-EGF), as a positive control of EGFR phophorylation, did not show differences between Cav1+/+ and Cav1−/− cells (Figure 3), to fully assess this result, we examined the response to other EGFR ligands such as EGF or transforming growth factor-alpha (TGF-α). Cav1 deficiency did not prevent the response to any of them in terms of receptor phosphorylation or activation of downstream signals (Figures 5a and b). Furthermore, Cav1 deficiency did not perturb either other cellular responses to EGF receptor ligands, such as cell proliferation (Figure 5c), despite Cav1−/− cells showed higher proliferative ratio in response to FBS, as we had previously described.16
Cav1 deficiency impairs TGF-β-mediated TACE/ADAM17 activity
Hepatocytes express different EGFR ligands, such as HB-EGF, TGF-α or Amphiregulin (Supplementary Figure 4). In order to know whether Cav1 may influence the expression of these ligands, we analyzed their transcript levels by real-time PCR. Results indicated that Cav1−/− cells do not show decreased expression of EGFR ligands. Even in the case of TGF-α, the levels were higher (Supplementary Figure 4). Indeed, we focused on the shedding of these EGFR ligands, which is required for the binding to the EGFR and activation of this pathway. We previously described that short-term activation of the metalloprotease TACE/ADAM17 by TGF-β is required for activation of the EGFR and pro-survival signals in hepatocytes.4 Taking this into consideration, here we wondered whether this pathway could be impaired in Cav1−/− hepatocytes. Analysis of TACE/ADAM17 enzymatic activity using a fluorimetric method demonstrated that Cav1+/+, but not Cav1−/−, hepatocytes responded to TGF-β increasing its activity (Figure 6a). Afterward, we studied whether this increase had functional consequences such as a decrease in the shedding of EGFR ligands into the cell media in TGF-β-treated Cav1−/− cells. To this aim, we collected conditioned media from FBS-starved Cav1+/+ and Cav1−/− hepatocytes, either untreated or treated with TGF-β to analyze their capacity to phosphorylate the EGFR in control Cav1+/+ cells. Conditioned media from Cav1+/+ cells treated with TGF-β induced the phosphorylation of the EGFR, in contrast to conditioned media from TGF-β-treated Cav1−/− cells (Figure 6b). Long-term treatment of Cav1+/+ hepatocytes with TGF-β increased TACE/ADAM17 mRNA levels (Figure 6c), which was dependent on the EGFR pathway, since knockdown of EGFR levels with a specific siRNA attenuated this response (Supplementary Figure 5). Upregulation of TACE by TGF-β was not observed in Cav1−/− hepatocytes (Figure 6c). Interestingly, Cav1 restitution in Cav1−/− hepatocytes led to a full recovery of TACE/ADAM17 activation after TGF-β treatment (Figure 6d), which reinforced and confirmed the importance of Cav1 in TACE/ADAM17 activity regulation.
TACE/ADAM17 in lipid rafts is necessary for its TGF-β-mediated activation
In search for a mechanism that explains how Cav1 may regulate TACE/ADAM17, we postulated colocalization of both proteins in lipid rafts of hepatocytes, as recently reported for vascular smooth muscle cells and endothelial cells.19, 20 Using a sucrose gradient technique to obtain detergent-resistant membranes (DRMs) fractions, we observed that a pool of TACE/ADAM17 is localized in DRMs (identified by the presence of ganglioside GM1) in Cav1+/+ hepatocytes (Figure 7a). This was not the case in Cav1−/− cells, where the amount of TACE/ADAM17 in DRMs is highly reduced (Figure 7a). This result demonstrates that Cav1 is necessary for TACE/ADAM17 localization in lipid rafts in hepatocytes. We also evaluated whether Cav1-dependent DRMs localization of TACE is regulated by TGF-β, particularly in stages when the metalloprotease is more active. We obtained DRMs from cells treated with TGF-β and observed an increase of TACE in DRMs in Cav1+/+ hepatocytes, but not in Cav1−/− cells (Figure 7a). Finally, as we found that TACE/ADAM17 localization in lipid rafts was increased after TGF-β treatment, we examined the effect of lipid raft disruption by cholesterol removal with methyl-β-cyclodextrin (MβC). MβC dramatically decreased TGF-β-induced TACE/ADAM17 activation in Cav1+/+ hepatocytes (Figure 7b), an effect that was reversed by treatment of cells with extracellular cholesterol (Supplementary Figure 6a). Furthermore, other milder cholesterol-binding agents, such as filipin III or nystatin, also inhibited TACE/ADAM17 activation by TGF-β (Supplementary Figure 6b). We conclude that Cav1 is required for TACE/ADAM17 activation upon localization to lipid rafts.
Discussion
The basic elements of the TGF-β pathway were detailed at the end of the last century. However, how the intracellular trafficking affects the TGF-β signal traveling from the cell membrane to the nucleus, how posttranslational regulation of receptors and Smads regulates signaling and/or how the context determines the cellular response to TGF-β are open questions yet.7, 21, 22, 23 In hepatocytes, TGF-β induces both pro- and anti-apoptotic signals, the latter mediated by the EGFR pathway, which is transactivated by TGF-β through a mechanism that involves upregulation of the EGFR ligands and activation of the metalloprotease TACE/ADAM17 responsible for their shedding.4, 5 Cav1, the major component of caveolae, had been also suggested to be necessary for TGF-β induction of anti-apoptotic signaling in hepatocytes, although the specific molecular mechanism is not well understood yet.10, 11 In this work, we demonstrate that in immortalized neonatal mice hepatocytes, Cav1 is required for TGF-β-mediated transactivation of the EGFR that initiates intracellular signal cascades, such as MAPKs/ERKs and PI-3K/AKT pathways, which directly prevent TGF-β-induced upregulation of the NADPH oxidase NOX4, which is required for its pro-apoptotic response.5 Indeed, failure in transactivation of the EGFR pathway increases ROS production and cell death.17 The molecular mechanism requires the participation of the TACE/ADAM17, a metalloprotease responsible for the shedding of the EGFR ligands.24 Our results indicate that Cav1 determines TACE/ADAM17 localization in lipid rafts in hepatocytes, which in turn is necessary for the TGF-β transactivation of the EGFR. Different lines of evidence support our conclusions: (1) in immortalized Cav1−/− hepatocytes, transactivation of the EGFR pathway by TGF-β is reduced, which correlates with the lack of activation of TACE/ADAM17; (2) the restitution of Cav1 in Cav1−/− hepatocytes recapitulates wild-type phenotype features both in terms of EGFR transactivation and TACE/ADAM17 activation; (3) TACE/ADAM17 localizes to DRMs; (4) the localization of TACE/ADAM17 in DRMs increases in response to TGF-β only in the presence of Cav1. TGF-β-dependent ERK1/2 and AKT phosphorylation was decreased in Cav1−/− cells, although extracellular EGFR ligand-dependent ERKs and AKT phosphorylation was even increased in Cav1−/− cells. Furthermore, basal expression of EGFR ligands is not lower in Cav1−/− cells. This corroborates and strengthens our conclusion that it is the TACE/ADAM17-dependent EGFR ligand shedding what is highly sensitive to Cav1 expression.
In support of the hypothesis that Cav1 is required for TACE/ADAM17 activation, it was suggested that its activity could be regulated by cholesterol in the plasma membrane.25 Our results after treatment with cholesterol scavengers (MβC, filipin III or nystatin), confirm this hypothesis and therefore are demonstrative that the integrity of lipid rafts is indeed necessary for TACE/ADAM17 activity. However, lipid raft-independent functions for TACE/ADAM17 have been also proposed.26 The requirement of cholesterol-rich domains for TACE/ADAM17 function could be cell type dependent and/or it could depend on the protein substrate.
Another important indirect conclusion of our study is that Cav1 is not required for the mitogenic response of hepatocytes to EGFR ligands such as EGF, TGF-α or HB-EGF. Pioneer studies in the nineties suggested that a cross talk between the EGFR and caveolins could govern EGFR signals. Activation of the EGFR was localized in non-caveolar membrane domains.27 However, EGFRs are initially concentrated in caveolae and rapidly move out of this membrane domain in response to EGFR ligands.28 Caveolin was proposed to be an inhibitor of the kinase domain of the EGFR, thus impairing its signaling.29 Caveolae do not appear to be involved in the endocytosis of EGF, which is mostly clathrin dependent,30 although recent works suggest that it could be also internalized by other pathways.31 Sustained evidence suggests that EGF stimulates the caveolae/raft-dependent endocytic pathway, which has been proposed to have relevant consequences on other cellular signals.14, 32 In human tumor cells, EGFR-mediated caveolae-dependent endocytosis disrupts cell–cell adhesion and causes EMT due to E-cadherin endocytosis33 and regulates integrin-mediated cell behavior through internalization and segregation into different intracellular compartments of integrins.34 The results presented in this study suggest that cell responses to EGFR ligands in terms of receptor phosphorylation, cell proliferation and survival are Cav1 independent in hepatocytes. However, further work is required for a detailed analysis of the role of Cav1 on other cell responses related to the pro-tumorigenic effects of the EGFR pathway and that will require the use of liver tumor cells in the study.
Although the role of Cav1 in cancer is controversial, its status has been proposed to be one of the causative factors for the invasion and poor prognosis of liver tumors, in particular hepatocellular carcinoma (HCC).35, 36 Overexpression of Cav1 is observed along HCC progression and could contribute to promote tumor growth and metastatic potential, through increase in tumor cell motility and invasiveness.36 Interestingly, well differentiated cell lines exhibit low and TGF-β-inducible Cav1 protein levels, whereas poorly differentiated and highly invasive HCC cell lines show high Cav1 expression.37 Poorly differentiated HCC cells show autocrine production of TGF-β, which is responsible for its migratory and invasive capacity.38 The results presented in this manuscript suggest that high expression of Cav1 in liver tumor cells would switch the response to TGF-β from a suppressor to a tumorigenic factor. Indeed, Cav1/lipid rafts are required for the activation of TACE/ADAM17 by TGF-β, which in turn activates the EGFR, whose signal transduction pathways compensate its anti-mitogenic and pro-apoptotic signals, allowing cells to respond to its pro-tumorigenic effects.4, 18, 39 We previously reported that TACE/ADAM17 activation is a requirement for TGF-β-induced transactivation of the EGFR in hepatocytes. Although TGF-β induces TGF-α and HB-EGF in adult rat hepatocytes, they show a poor response to this cytokine in terms of EGFR transactivation because expression of TACE/ADAM17 is very low in these cells and the shedding/activation of the EGFR ligands is prevented. In contrast, liver tumor cells express high levels of TACE/ADAM17 and show higher capacity to transactivate the EGFR pathway in response to TGF-β.40 Due to this differential response, TGF-β is a suppressor factor for adult quiescent hepatocytes, but not for hepatoma cells, where it plays a dual role, both suppressing and promoting carcinogenesis. Importantly, inhibition of TACE/ADAM17 in fetal hepatocytes or in hepatoma cells switch the TGF-β function toward apoptosis and growth suppression.4, 40 As TACE/ADAM17 is also required for TGF-β-induced growth/survival signals in breast cancer41, 42 or gastric cancer43 cells, as well as for promotion of motility and invasiveness by TGF-β in glioma cells,44 the mechanism described in this work could have transcendence in the pro-tumorigenic effects that TGF-β exerts in many other tumors.
Materials and Methods
Reagents and antibodies
Human recombinant TGF-β1 and AG1478 were from Calbiochem (La Jolla, CA, USA). EGF was kindly gifted by Serono Lab (Madrid, Spain). Human recombinant HB-EGF, human recombinant TGF-α, MβC, filipin III from Streptomyces filipinensis, nystatin and water-soluble cholesterol were from Sigma-Aldrich (St. Louis, MO, USA). The antibodies used were: mouse anti-β-actin (clone AC-15) from Sigma-Aldrich, rabbit anti-phospho-Akt (Ser473) (D9E) XP, rabbit anti-Akt, rabbit anti-phospho-EGFR (Tyr1068) (D7A5) XP, rabbit anti-EGFR, rabbit anti-phospho-p44/42 MAPK (Thr202/Tyr204), rabbit anti-p44/42 MAPK were from Cell Signaling Technology (Beverly, MA, USA), mouse anti-Caveolin-1 from BD Biosciences (Franklin Lakes, NJ, USA), rabbit anti-Ki67 from AbCam (Cambridge, UK), rabbit anti-TACE/ADAM17 (807-823) from Calbiochem, rabbit anti-NFκB p65, rabbit anti-TβRI (H-100, used in immunocytochemistry), rabbit anti-TβRI (R-20, used in western blot) and rabbit anti-TβRII (C-16) from Santa Cruz Biotechnologies (Dallas, TX, USA). Secondary antibodies: Alexa Fluor 488-conjugated anti-rabbit and anti-mouse from Molecular Probes (Eugene, OR, USA) and ECL Mouse IgG, and Rabbit IgG, HRP-Linked antibodies from GE Healthcare (Buckinghamshire, UK). GM1 was detected with horseradish peroxidase-tagged cholera toxin B subunit from Sigma (St. Louis, MO, USA).
Cell culture conditions
Immortalized neonatal hepatocytes from wild type (Cav1+/+) and caveolin-1 knockout (Cav1−/−) mice were kindly provided by Paloma Martín-Sanz (IIBM Alberto Sols, Madrid, Spain). Isolation and immortalization of neonatal hepatocytes from Cav1+/+ and Cav1−/− mice has been previously described.16, 45, 46 In order to validate our experimental model, we confirmed that Cav1 knockout cell line does not express Cav1 (Supplementary Figures 1a and d). We also tested that both cell lines showed no differences in the expression of the TGF-β receptors type 1 and 2 (Supplementary Figures 1b–d). Reconstitution of Cav1−/− cell line (Cav1−/− +IRES-GFP and Cav1−/− +Cav1) was kindly performed in Miguel A. del Pozo Lab (CNIC, Madrid, Spain) as previously described.47 For cell culture, cells were grown in DMEM from Lonza (Basel, Switzerland), supplemented with 10% fetal bovine serum (FBS), penicillin (120 μg/ml), streptomycin (100 μg/ml) and amfoterycin B (2.5 μg/ml) and maintained in a humidified atmosphere of 37 °C, 5% CO2. For experiments, cells at 60% confluence were serum-starved for 16 h and treated with different factors: TGF-β1 (2 ng/ml), EGF (20 ng/ml), HB-EGF (20 ng/ml) and TGF-α (20 ng/ml). When indicated, the following products were added 30 min before the other factors (TGF-β1 or HB-EGF) and maintained along the experiment: AG1478 (20 μM), MβC (2.5 μM), filipin III (0.5 μM) and nystatin (25 μM). For reversion of the MβC effect by treatment of cells with extracellular cholesterol (1 mM), this lipid was added after 30-min treatment with MβC, waiting for an additional 1 h before adding TGF-β1, and maintained along the experiment.
Analysis of cell viability
Cell viability was analyzed using both trypan blue and propidium iodide (PI). For trypan blue dye cells were trypsinized collecting the media. After 5-min centrifugation at 1200 r.p.m., cells were resuspended in 50 μl PBS+1 : 10 v/v trypan blue. Viable and non-viable (blue dyed) cells were counted in a Neubauer chamber, counting eight squares for condition, with duplicates. Results are expressed as percentage of viable and non-viable cells. For PI staining, 1 μg/ml (final concentration) PI from Sigma-Aldrich was added to each well (from a 12-well multiwell plate). Afterward, PI-positive cells were counted in a phase contrast microscope Olympus IX70 (Tokyo, Japan). Results were expressed as percentage of PI-positive cells.
Measurement of intracellular redox state
The oxidation-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA; from Invitrogen, Carlsbad, CA, USA) was used to analyze the total intracellular content of ROS as previously described.39 Fluorescence was measured in a Microplate Fluorescence Reader Fluostar Optima (BMG LABTECH, Ortenberg, Germany) and expressed as percentage of control after correction with protein content.
Analysis of caspase-3 activity
Fluorimetric analysis of caspase-3 activity was determined as described previously,4 using the Caspase-3 fluorogenic substrate Ac-DEVD-AMC from BD Pharmingen (Franklin Lakes, NJ, USA), with 20 μg of protein extract. Protein concentration of cell lysates was determined using Bradford Reagent from Sigma-Aldrich. A unit of caspase-3 activity is the amount of active enzyme necessary to produce an increase in 1 fluorescence unit and results are presented as units of caspase-3 activity/h/μg protein.
Western blot analysis
Total protein extracts and western blot procedure were carried out as previously described.5, 39 Primary antibodies were used at 1 : 1000, except β-actin (1 : 5000), secondary antibodies were used at 1 : 5000. Protein concentration was measured with BCA Protein Assay kit (Pierce, Rockford, USA). Densitometric analysis of protein bands intensity was performed using ImageJ software (Public domain, NIH, Bethesda, MD, USA).
Dot blot analysis
A 2-μl drop of protein extract is applied onto nitrocellulose membrane and let to dry during 6–8 h. The following procedure is the same as for western blot analysis.
Immunocytochemistry studies
Fluorescence microscopy studies were performed as described previously.40 Cells were fixed with 4% paraformaldehyde in PBS for 20 min at RT for NFκB-p65 and Ki67 stainings and with methanol for 2 min at −20 °C for phospho-EGFR, TβRI, TβRII and caveolin-1 stainings. Coverslips were then incubated with primary antibody diluted in 1% BSA for 2 h at RT for NFκB-p65 and Ki67 or overnight at 4 °C for all other antibodies (dilutions: NFκB-p65 1 : 50; Ki67 1 : 100; p-EGFR 1 : 200; TβRI 1 : 200, TβRII 1 : 200 and caveolin-1 1 : 200). After several washes with PBS, the samples were incubated with Alexa Fluor 488-conjugated anti-rabbit or anti-mouse for 1 h at RT (1 : 200). After washing with PBS, nuclear DNA was stained with DAPI (Sigma-Aldrich) and samples were mounted in MOWIOL 4-88 reagent (Calbiochem). Cells were visualized in a Nikon-80I microscope (Tokyo, Japan) with the appropriate filters, except in the Supplementary Figure 1, where an Olympus BX-60 microscope (Tokyo, Japan) and a Leica TCS-NT confocal microscope (Wetzlar, Germany) were used (as specified in its figure legend). Representative images were taken and edited in Adobe Photoshop software.
TACE/ADAM17 activity measurement
Fluorimetric measurement of TACE/ADAM17 activity was determined as described previously,4 using 10 μM TACE Substrate IV from Calbiochem, with 10 μg of protein extract. Fluorescence was measured in a Microplate Fluorescence Reader Fluostar Optima every 5 min during 1 h at 37 °C (excitation: 320 nm; emission: 420 nm). Protein concentration of cell lysates was determined using Bradford reagent from Sigma-Aldrich. Results were expressed as percentage of activity compared with the untreated control (100%).
Analysis of gene expression
RNeasy Mini Kit (Qiagen, Valencia, CA, USA) was used for total RNA isolation. Reverse transcription (RT) was carried out using the High Capacity Reverse Transcriptase kit (Applied Biosystems, Foster City, CA, USA), with 500 ng of total RNA from each sample for complementary DNA synthesis.
For real-time quantitative PCR, expression levels were determined in duplicate in an ABIPrism7700 System, using the Sybr Green PCR Master Mix (Applied Biosystems) or in a LightCycler 480 System, using LightCycler 480 SYBR Green I Master Mix (Roche, Basel, Switzerland). Reactions were performed with the following mouse-specific primers:
Knockdown assays
For transient siRNA transfection, cells were transfected at 50% confluence using TransIT-siQuest (Mirus, Madison, WI, USA) at 1 : 300 dilution in complete medium, according to the manufacturer’s recommendation, with a final siRNA concentration of 50 nM during 8 h. Oligos were obtained from Sigma-Genosys (Suffolk, UK). The oligo sequences were as follows:
Unsilencing 5′-GUAAGACACGACUUA UCGC-3′
Cav1(1) 5′-ACGUAGACUCCGAGGGACA-3′
Cav1(2) 5′-GCAAAUACGUAGACUCCGA-3′
EGFR 5′-GCGAUAAGUCGUGUCUUAC-3′
The unsilencing siRNA used was selected from previous works.39
Preparation of detergent-resistant membrane (DRM) fractions
DRM fractions were purified as described in Navarro-Lerida et al.48 Two confluent p100 plates were scraped from each cell line and resuspended in 335 μl (final volume) MES-buffered saline (MBS: 25 mM MES, pH 6.5, 0.15 M NaCl, 1 mM PMSF plus 1% Triton X-100) at 4 °C. Cells were homogenized by a minimum of 10 strokes through a syringe (0.5 × 16 mm) on ice. The homogenate was brought up to 670 μl by adding 335 μl 80% sucrose in MBS, and placed at the bottom of a Beckman TLS-55 2.2 ml Ultraclear tube (Beckman Coulter, Brea, CA, USA). The discontinuous sucrose gradient (40–30–5%) was formed by sequentially loading 670 μl 30% sucrose and 670 μl 5% sucrose in MBS. Separation was performed by centrifuging at 50 000 r.p.m. for 3 h in a TLS-55 rotor (Beckman Coulter) at 4 °C (using an Optima MAX-TL ultracentrifuge, Beckman Coulter). A light, scattered band confined to the 5–30% sucrose interface was observed that contained most caveolin-1 protein and excluded most other proteins. Six 335-μl fractions were collected, starting from the bottom of the tube. An equal volume of cold acetone was added to each fraction and the proteins precipitated overnight at 4 °C. Protein pellets were collected by centrifugation at 13 000 r.p.m. and air-dried for 2 h to eliminate acetone traces. The protein precipitates were then analyzed by SDS-PAGE and western blot, or dot blot in the case of GM1.
Statistics
All data represents at least three experiments and are expressed as the mean±S.E.M. Differences between groups were compared using Student’s t-test (when comparing two groups), one-way ANOVA with Tukey’s multiple comparison test (when comparing more than two groups and considering one independent variable) or two-way ANOVA with Bonferroni post hoc test (when comparing differences between groups considering two independent variables). All statistical tests were conducted using GraphPad Prism (La Jolla, CA, USA). Differences were considered statistically significant at *P<0.05, **P<0.01 and ***P<0.001.
Abbreviations
- Cav1:
-
caveolin-1
- DRMs:
-
detergent-resistant membranes
- EGF:
-
epidermal growth factor
- EGFR:
-
epidermal growth factor receptor
- HB-EGF:
-
heparin-binding EGF-like growth factor
- MβC:
-
methyl-β-cyclodextrin
- NOX:
-
NADPH oxidase
- PI-3K:
-
phospho-inositol-3-kinase
- ROS:
-
reactive oxygen species
- TGF-α:
-
transforming growth factor-alpha
- TGF-β:
-
transforming growth factor-beta
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Acknowledgements
This work was supported by grants from: (1) the Ministry of Economy and Competitiveness (MINECO), Spain (BFU2012-35538 and ISCIII-RTICC: RD12-0036-0029 to IF; SAF2013-43713 to PM-S; BFU2012-33932 to GE; SAF2011-25047 and CSD2009-00016 to MAdP); (2) AGAUR-Generalitat de Catalunya (2009SGR-312 to IF); and (3) People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. PITN-GA-2012-316549 (IT LIVER) to IF JM-C and RM-V were recipients of pre-doctoral fellowships from the FPU program (Ministry of Education, Culture and Sport, Spain) and the FPI program (associated to SAF2011-25047, MINECO, Spain), respectively. We acknowledge the review and suggestions of Dr. Christoph Meyer (University of Heidelberg, Mannheim, Germany).
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Moreno-Càceres, J., Caja, L., Mainez, J. et al. Caveolin-1 is required for TGF- β -induced transactivation of the EGF receptor pathway in hepatocytes through the activation of the metalloprotease TACE/ADAM17 . Cell Death Dis 5, e1326 (2014). https://doi.org/10.1038/cddis.2014.294
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DOI: https://doi.org/10.1038/cddis.2014.294
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