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Is TALL-1 a trimer or a virus-like cluster?

Abstract

Native TALL-1 (B-cell activation factor, BAFF; also known as BlyS) was initially described as a homotrimer1,2,3, but Liu and colleagues claim that it is a 60-subunit complex4,5,6 on the basis of their results from X-ray crystallography and size-exclusion chromatography. They consider TALL-1 60-mers to be the biologically active form, and the arrangement of the 60-mers resembles that of the capsid of satellite tobacco necrosis virus4,5,6. Here we show that active TALL-1 is trimeric under normal physiological conditions and that formation of higher-order oligomers is an artefact of tagging the amino terminus of the protein with a histidine tag.

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Figure 1: Effect of different amino-terminal tags on the oligomerization and biological properties of TALL-1.

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References

  1. Kanakaraj, P. et al. Cytokine 12, 25–31 (2001).

    Article  Google Scholar 

  2. Karpusas, M. et al. J. Mol. Biol. 315, 1145–1154 (2002).

    Article  CAS  Google Scholar 

  3. Oren, D. A. et al. Nature Struct. Biol. 9, 288–292 (2002).

    Article  CAS  Google Scholar 

  4. Liu, Y. et al. Cell 108, 383–394 (2002).

    Article  CAS  Google Scholar 

  5. Liu, Y., et al. Nature 423, 49–56 (2003).

    Article  ADS  CAS  Google Scholar 

  6. Kim, H. M. et al. Nature Struct. Biol. 10, 342–348 (2003).

    Article  CAS  Google Scholar 

  7. Wu, J. & Filutowicz, M. Acta Biochim. Pol. 46, 591–599 (1999).

    CAS  PubMed  Google Scholar 

  8. Arunkumar, A. et al. Protein Sci. 11, 1050–1061 (2002).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Eugene A. Zhukovsky.

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Zhukovsky, E., Lee, JO., Villegas, M. et al. Is TALL-1 a trimer or a virus-like cluster?. Nature 427, 413–414 (2004). https://doi.org/10.1038/427413a

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