Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Progress
  • Published:

Gene inhibition using antisense oligodeoxynucleotides

Nature volume 372, pages 333–335 (1994)Cite this article

Abstract

Antisense oligodeoxynucleotides (ODNs) have great promise as agents for the specific manipulation of gene expression. Until recently, nonspecific effects of ODNs often confounded the interpretation of antisense studies. Improvements in ODN chemistry and cellular delivery techniques now allow for more potent and specific gene inhibition. This review critically evaluates recent progress in the development of antisense ODNs.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Milligan, J. F., Matteucci, M. D. & Martin, J. C. J. med. Chem. 36, 1923–1937 (1993).

    Article  CAS  Google Scholar 

  2. Stein, C. A. & Cheng, Y.-C. Science 261, 1004–1012 (1993).

    Article  ADS  CAS  Google Scholar 

  3. Helene, C. & Toulme, J. Biochim. biophys. Acta 1049, 99–125 (1990).

    Article  CAS  Google Scholar 

  4. Abe, J. et al. Biochem. biophys. Res. Commun. 198, 16–24 (1994).

    Article  CAS  Google Scholar 

  5. Amaratunga, A., Morin, P. J., Kosik, K. S. & Fine, R. E. J. biol. Chem. 268, 17427–17430 (1993).

    CAS  PubMed  Google Scholar 

  6. Bayever, E. et al. Antisense Res. Dev. 3, 383–390 (1993).

    Article  CAS  Google Scholar 

  7. Bennett, M. R. et al. J. clin. Invest. 93, 820–828 (1994).

    Article  CAS  Google Scholar 

  8. Chiasson, B. J., Hooper, M. L., Murphy, P. R. & Robertson, H. A. Eur. J. Pharmac. 227, 451–453 (1992).

    Article  CAS  Google Scholar 

  9. Kitajima, I. et al. J. biol. Chem. 267, 25881–25888 (1992).

    CAS  PubMed  Google Scholar 

  10. Kitajima, I. et al. Science 258, 1792–1795 (1992).

    Article  ADS  CAS  Google Scholar 

  11. McCarthy, M. M., Masters, D. B., Rimvall, K., Schwartz-Giblin, S. & Pfaff, D. W. Brain Res. 636, 55–58 (1994).

    Article  Google Scholar 

  12. Morishita, R. et al. Proc. natn. Acad. Sci. U.S.A. 90, 8474–8478 (1993).

    Article  ADS  CAS  Google Scholar 

  13. Morishita, R. et al. J. clin. Invest 93, 1458–1464 (1994).

    Article  CAS  Google Scholar 

  14. Offensperger, W. B. et al. EMBO J. 12, 1257–1262 (1993).

    Article  CAS  Google Scholar 

  15. Osen-Sand, A. et al. Nature 364, 445–448 (1993).

    Article  ADS  CAS  Google Scholar 

  16. Perlaky, L. et al. Anticancer Drug Des. 8, 3–14 (1993).

    CAS  PubMed  Google Scholar 

  17. Simons, M., Edelman, E. R., De Keyser, J. L., Langer, R. & Rosenberg, R. D. Nature 359, 67–70 (1992).

    Article  ADS  CAS  Google Scholar 

  18. Simons, M., Edelman, E. R. & Rosenberg, R. D. J. clin. Invest. 93, 2351–2356 (1994).

    Article  CAS  Google Scholar 

  19. Skutella, T., Probst, J. C., Jirikowski, G. F., Holsboer, F. & Spanagel, R. Neurosci. Lett. 167, 55–58 (1994).

    Article  CAS  Google Scholar 

  20. Standifer, K. M., Chien, C. C., Wahlestedt, C., Brown, G. P. & Pasternak, G. W. Neuron 12, 805–810 (1994).

    Article  CAS  Google Scholar 

  21. Whitesell, L., Rosolen, A. & Neckers, L. M. Antisense Res. Dev. 1, 343–350 (1991).

    Article  CAS  Google Scholar 

  22. Zhou, L. W., Zhang, S. P., Qin, Z. H. & Weiss, B. J. pharmac. exp. Ther. 268, 1015–1023 (1994).

    CAS  Google Scholar 

  23. Higgins, K. A. et al. Proc. natn. Acad. Sci. U.S.A. 90, 9901–9905 (1993).

    Article  ADS  CAS  Google Scholar 

  24. Hijiya, N. et al. Proc. natn. Acad. Sci. U.S.A. 91, 4499–4503 (1994).

    Article  ADS  CAS  Google Scholar 

  25. Skorski, T. et al. Proc. natn. Acad. Sci. U.S.A. 91, 4504–4508 (1994).

    Article  ADS  CAS  Google Scholar 

  26. Shi, Y. et al. Circulation 90, 944–951 (1994).

    Article  CAS  Google Scholar 

  27. Alper, J. Biotechnology 11, 1225 (1993).

    Google Scholar 

  28. Maister, P. Bioworld Today 5, 3 (1994).

    Google Scholar 

  29. Woolf, T. M., Melton, D. A. & Jennings, C. G. Proc. natn. Acad. Sci. U.S.A. 89, 7305–7309 (1992).

    Article  ADS  CAS  Google Scholar 

  30. Ellington, A. D. & Szostak, J. W. Nature 346, 818–822 (1990).

    Article  ADS  CAS  Google Scholar 

  31. Gao, W.-Y., Han, F.-S., Storm, C., Egan, W. & Cheng, Y.-C. Molec. Pharmac. 41, 223–229 (1992).

    CAS  Google Scholar 

  32. Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H. & Toole, J. J. Nature 355, 564–566 (1992).

    Article  ADS  CAS  Google Scholar 

  33. Yakubov, L. et al. J. biol. Chem. 268, 18818–18823 (1993).

    CAS  PubMed  Google Scholar 

  34. Perez, J. R. et al. Proc. natn. Acad. Sci. U.S.A. 91, 5957–5961 (1994).

    Article  ADS  CAS  Google Scholar 

  35. Yao, G.-Q., Grill, S., Egan, W. & Cheng, Y.-C. Antimicrob. Agents Chemother. 37, 1420–1425 (1993).

    Article  CAS  Google Scholar 

  36. Azad, R. F., Driver, V. B., Tanaka, K., Crooke, R. M. & Anderson, K. P. Antimicrob. Agents Chemother. 37, 1945–1954 (1993).

    Article  CAS  Google Scholar 

  37. Rathbone, M. P. et al. In Vitro Cell dev. Biol. 28A, 529–536 (1992).

    Article  CAS  Google Scholar 

  38. Kamano, H. et al. Biochem. Int. 26, 537–543 (1992).

    CAS  PubMed  Google Scholar 

  39. Shaw, J. P., Kent, K., Bird, J., Fishback, J. & Froehler, B. Nucleic Acids Res. 19, 747–750 (1991).

    Article  CAS  Google Scholar 

  40. Fisher, T. L., Terhorst, T., Cao, X. & Wagner, R. W. Nucleic Acids Res. 21, 3857–3865 (1993).

    Article  CAS  Google Scholar 

  41. Agrawal, S., Temsamani, J. & Tang, J. Y. Proc. natn. Acad. Sci. U.S.A. 88, 7595–7599 (1991).

    Article  ADS  CAS  Google Scholar 

  42. Wagner, R. W. et al. Science 260, 1510–1513 (1993).

    Article  ADS  CAS  Google Scholar 

  43. Kirkland, M. A. et al. Lancet 342, 614 (1993).

    Article  CAS  Google Scholar 

  44. Stein, C. A. Leukemia 6, 967–974 (1992).

    CAS  PubMed  Google Scholar 

  45. Dean, N. M., McKay, R., Condon, T. P. & Bennett, C. F. J. biol. Chem. 269, 16416–16424 (1994).

    CAS  PubMed  Google Scholar 

  46. Bennett, C. F., Condon, T. P., Grimm, S., Chan, H. & Chiang, M.-Y. J. Immun. 152, 3530–3540 (1994).

    CAS  PubMed  Google Scholar 

  47. Fenster, S. D., Wagner, R. W., Froehler, B. C. & Chin, D. J. Biochemistry 33, 8391–8398 (1994).

    Article  CAS  Google Scholar 

  48. Bennett, C. F., Chiang, M. Y., Chan, H., Shoemaker, J. E. & Mirabelli, C. K. Molec. Pharmac. 41, 1023–1033 (1992).

    CAS  Google Scholar 

  49. Colige, A., Sokolov, B. P., Nugent, P., Baserga, R. & Prockop, D. J. Biochemistry 32, 7–11 (1993).

    Article  CAS  Google Scholar 

  50. Monia, B. P. et al. J. biol. Chem. 268, 14514–14522 (1993).

    CAS  PubMed  Google Scholar 

  51. Epstein, S. E., Speir, E. & Finkel, T. Circulation 88, 1351–1353 (1993).

    Article  CAS  Google Scholar 

  52. Chiang, M. Y. et al. J. biol. Chem. 266, 18162–18171 (1991).

    CAS  PubMed  Google Scholar 

  53. Piatak, J. M. et al. Science 259, 1749–1754 (1993).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Gilead Sciences, 353 Lakeside Drive, Foster City, California, 94404, USA

    Richard W. Wagner

Authors
  1. Richard W. Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wagner, R. Gene inhibition using antisense oligodeoxynucleotides. Nature 372, 333–335 (1994). https://doi.org/10.1038/372333a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/372333a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing