Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor

Nature volume 364, pages 343–346 (1993)Cite this article

Abstract

MACROPHAGES interact with other cells and components of the extracellular environment by means of adhesion receptors1,2. Adhesion to artificial substrata in vitro facilitates isolation of macrophages3, and has been used to generate antibodies that inhibit their migration in vivo4,5. Unlike other cell types, macrophages attach to tissue culture plastic in the absence of divalent cations. Here we use an adhesion assay exploiting this property to isolate a rat monoclonal antibody, 2F8, which totally inhibits divalent cation-independent adhesion of murine macrophages to tissue culture plastic in the presence of fetal calf serum. Immunoprecipitation from macrophages and stably transfected Chinese hamster ovary cells revealed that the antigen recognized by monoclonal 2F8 is identical to murine macrophage scavenger receptor6,7. We propose a novel function for this molecule, previously described as an endocytic receptor, thus providing a mechanism for mononuclear phagocyte recruitment to and retention in ligand-rich tissues such as in atherosclerotic lesions.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Pearlstein, E., Dienstman, S. R. & Defendi, V. J. Cell Biol. 79, 263–267 (1978).

    Article  CAS  Google Scholar 

  2. Springer, T. A. & Anderson, D. C. Ciba Found. Symp, 118, 102–126 (1986).

    CAS  PubMed  Google Scholar 

  3. Johnson, W. D. Jr Mei, B. & Cohn, Z. A. J. exp. Med. 146, 1613–1626 (1977).

    Article  CAS  Google Scholar 

  4. Rosen, H. & Gordon, S. J. exp. Med. 166, 1685–1701 (1987).

    Article  CAS  Google Scholar 

  5. Rosen, H., Gordon, S. & North, R. J. J. exp. Med. 170, 27–37 (1989).

    Article  CAS  Google Scholar 

  6. Krieger, M. Trends biochem. Sci. 17, 141–146 (1992).

    Article  CAS  Google Scholar 

  7. Freeman, M. et al. Proc. natn. Acad. Sci. U.S.A. 87, 8810–8814 (1990).

    Article  ADS  CAS  Google Scholar 

  8. Hynes, R. O. Cell 69, 11–25 (1992).

    Article  CAS  Google Scholar 

  9. Bevilacqua, M. et al. Cell 67, 233 (1991).

    Article  CAS  Google Scholar 

  10. Springer, T. A. Nature 346, 425–434 (1990).

    Article  ADS  CAS  Google Scholar 

  11. Leenen, P. J. M., Jansen, A. M. A. C. & van Ewijk, W. Differentiation 32, 157–164 (1986).

    Article  CAS  Google Scholar 

  12. Raschke, W. C., Baird, S., Ralph, P. & Nakoinz, I. Cell 15, 261–267 (1978).

    Article  CAS  Google Scholar 

  13. Galfre, G., Milstein, C. & Wright, B. Nature 277, 131–133 (1979).

    Article  ADS  CAS  Google Scholar 

  14. Penman, M. et al. J. biol. Chem. 266, 23985–23993 (1991).

    CAS  PubMed  Google Scholar 

  15. Ashkenas, J. et al. J. Lipid Res. 34, 983–1000 (1993).

    CAS  PubMed  Google Scholar 

  16. Davis, G. E. Expl Cell Res. 200, 242–252 (1992).

    Article  ADS  CAS  Google Scholar 

  17. Quinn, M. T., Parthasarathy, S., Fong, L. G. & Steinberg, D. Proc. natn. Acad. Sci. U.S.A. 84, 2995–2998 (1987).

    Article  ADS  CAS  Google Scholar 

  18. Hale, G. et al. J. immun. Meth. 103, 59–67 (1987).

    Article  CAS  Google Scholar 

  19. Goding, J. W. in Handbook of Experimental Immunology (eds Weir, D. M.) 20.1–20.33 (Blackwell Scientific, Oxford, 1986).

    Google Scholar 

  20. Laemmli, U.K. Nature 227, 680–681 (1970).

    Article  ADS  CAS  Google Scholar 

  21. Geng, Y.-j. & Hansson, G. K. J. clin. Invest. 89, 1322–1330 (1992).

    Article  CAS  Google Scholar 

  22. Pitas, R. E., Innerarity, T. L., Weinstein, J. N. & Mahley, R.W. Arteriosclerosis 1, 177–185 (1981).

    Article  CAS  Google Scholar 

  23. Rabinovitch, M. & de Stefano, M. J. J. exp. Med. 143, 290–304 (1976).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, 0X13RE, UK

    Iain Fraser, Derralynn Hughes & Siamon Gordon

Authors
  1. Iain Fraser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Derralynn Hughes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Siamon Gordon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fraser, I., Hughes, D. & Gordon, S. Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor. Nature 364, 343–346 (1993). https://doi.org/10.1038/364343a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/364343a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing