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Regulation of Ca2+ -dependent K+ -channel activity in tracheal myocytes by phosphorylation

Abstract

ISOPRENALINE is a (β-adrenergic agonist of clinical importance as a remedy for asthma. In airway smooth muscle its relaxant action is accompanied by hyperpolarization of the membrane1,2 and elevation of the level of intracellular cyclic AMP3. Hyper-polarization and relaxation are also induced by drugs such as forskolin, theophylline and dibutyryl cAMP, indicating that cAMP-dependent phosphorylation is involved in producing the electrical response1. Cyclic AMP-dependent protein kinase (pro-tein kinase A) has been reported to activate Ca2+-dependent K+ channels in cultured aortic smooth muscle cells4 and snail neurons5,6. The membrane of tracheal smooth-muscle cells is characterized by a dense distribution of Ca2+-dependent K+-channels7. We have now examined the effect of isoprenaline and protein kinase A on Ca2+-dependent K+-channels in isolated smooth muscle cells of rabbit trachea, using the patch-clamp technique8. Our results show that the open-state probability of Ca2+-dependent K+-channel of tracheal myocytes is reversibly increased by either extracellular application of isoprenaline or intracellar application of protein kinase A. We also show that this effect is significantly enhanced and prolonged in the presence of a potent protein phos-phatase inhibitor, okadaic acid9–13.

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References

  1. Honda, K., Satake, T., Takagi, K. & Tomita, T. Br. J. Pharmac. 87, 665–671 (1986).

    Article  CAS  Google Scholar 

  2. Honda, K. & Tomita, T. Jap. J. Physiol. 37 333–336 (1987).

    Article  CAS  Google Scholar 

  3. Fredholm, B. B., Brodin, K. & Standberg, K. Acta pharmac. tox. 45, 336–344 (1979).

    Article  CAS  Google Scholar 

  4. Sadoshima, J., Akaike, N., Kanaide, H. & Nakamura, M. Am. J. Physiol. 255, H754–H759 (1988).

    Article  CAS  Google Scholar 

  5. De Peye, J. E., Cachelin, A. B., Levitan, I. B. & Reuter, H. Proc. natn. Acad. Sci. U.S.A. 79, 4207–4211 (1982).

    Article  ADS  Google Scholar 

  6. Ewald, D. A., Williams, A. & Levitan, I. B. Nature 315, 503–506 (1985).

    Article  ADS  CAS  Google Scholar 

  7. MacCann, J. & Walsh, M. J. J. Physiol., Lond. 372, 264–270 (1986).

    Google Scholar 

  8. Hamill, O. P., Marty, A., Neher, E., Sackmann, B. & Sigworth, J. Pflügers Arch. ges. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  9. Takai, A., Bialojan, C., Troschka, M. & Rüegg, J. C. FEBS Lett., 217, 81–84 (1987).

    Article  CAS  Google Scholar 

  10. Bialojan, C., Takai, A. & Rüegg, J. C. J. Physiol., London 398, 81–95 (1988).

    Article  CAS  Google Scholar 

  11. Hescheler, J., Mieskes, G., Rüegg, J. C., Takai, A. & Trautwein, W. Pflügers Arch. ges. Physiol. 412, 248–252 (1988).

    Article  CAS  Google Scholar 

  12. Bialojan, C. & Takai, A. Biochem. J. 256, 283–290 (1988).

    Article  CAS  Google Scholar 

  13. Takai, A. J. Muscle Res. Cell Motil. 9, 563–565 (1988).

    Article  CAS  Google Scholar 

  14. Benham, C. D., Bolton, T. B., Lang, R. J. & Takewaki, T. J. Physiol., Lond. 371, 45–67 (1986).

    Article  CAS  Google Scholar 

  15. Tachibana, K. et al. J. Am. chem. Soc. 103, 2469–2471 (1981).

    Article  CAS  Google Scholar 

  16. Ingebritsen, T.S. & Cohen, P. Eur. J. Biochem. 132, 255–261 (1983).

    Article  CAS  Google Scholar 

  17. Haystead, T.A. et al. Nature 337, 78–81 (1989).

    Article  ADS  CAS  Google Scholar 

  18. Kodama, I., Kondo, N. & Shibata, S. J. Physiol., Lond. 378, 359–373 (1986).

    Article  CAS  Google Scholar 

  19. Reuter, H. Nature 301, 569–574 (1983).

    Article  ADS  CAS  Google Scholar 

  20. Suganuma, M. et al. Proc. natn. Acad. Sci. U.S.A. 85, 1768–1771 (1988).

    Article  ADS  CAS  Google Scholar 

  21. Takai, A., Troschka, M., Mieskes, G. & Somlyo, A. Biochem. J. (in the press).

  22. Inoue, R., Kitamura, K. & Kuriyama, H. Pflügers Arch. ges. Physiol. 405, 173–179 (1985).

    Article  CAS  Google Scholar 

  23. Hardman, J. G. in Smooth Muscle: An Assessment of Current Knowledge (eds Bülbring, E., Brading, A. F., Jones, A. W. & Tomita, T.) 249–262 (1981).

    Google Scholar 

  24. Hisayama, T. & Takayanagi, I. Biochem. Pharmac. 32, 3197–3203 (1983).

    Article  CAS  Google Scholar 

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Kume, H., Takai, A., Tokuno, H. et al. Regulation of Ca2+ -dependent K+ -channel activity in tracheal myocytes by phosphorylation. Nature 341, 152–154 (1989). https://doi.org/10.1038/341152a0

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