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Purification and reconstitution of the calcium release channel from skeletal muscle

Nature volume 331, pages 315–319 (1988)Cite this article

Abstract

The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet9 that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca22+-release channels.

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Author notes

  1. Harold P. Erickson: Department of Anatomy, Duke University Medical Center, Durham, North Carolina 27710, USA

Authors and Affiliations

  1. Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27599, USA

    F. Anthony Lai, Harold P. Erickson, Eric Rousseau, Qi-Yi Liu & Gerhard Meissner

Authors
  1. F. Anthony Lai
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  2. Harold P. Erickson
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  3. Eric Rousseau
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  4. Qi-Yi Liu
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  5. Gerhard Meissner
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Anthony Lai, F., Erickson, H., Rousseau, E. et al. Purification and reconstitution of the calcium release channel from skeletal muscle. Nature 331, 315–319 (1988). https://doi.org/10.1038/331315a0

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