Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Cloning and sequence analysis of cDNA for porcine β-neo-endorphin/dynorphin precursor

Abstract

The primary structure of a precursor protein that contains β-neo-endorphin, dynorphin and a third leucine-enkephalin sequence with a carboxyl extension has been deduced from the nucleotide sequence of cloned DNA complementary to the porcine hypothalamic mRNA encoding it. The three peptides are each bounded by Lys–Arg. This precursor protein, like adrenal preproenkephalin and the corticotropin/β-lipotropin precursor, comprises multiple repetitive units and a cysteine-containing ammo-terminal sequence preceded by a signal peptide.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hughes, J. et al. Nature 258, 577–579 (1975).

    Article  ADS  CAS  Google Scholar 

  2. Li, C. H. & Chung, D. Proc. natn. Acad. Sci. U.S.A. 73, 1145–1148 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Minamino, N., Kangawa, K., Chino, N., Sakakibara, S. & Matsuo, H. Biochem. biophys. Res. Commun. 99, 864–870 (1981).

    Article  CAS  Google Scholar 

  4. Goldstein, A., Fischli, W., Lowney, L. I., Hunkapiller, M. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 78, 7219–7223 (1981).

    Article  ADS  CAS  Google Scholar 

  5. Tachibana, S., Araki, K., Ohya, S. & Yoshida, S. Nature 295, 339–340 (1982).

    Article  ADS  CAS  Google Scholar 

  6. Kita, T., Inoue, A., Nakanishi, S. & Numa, S. Eur. J. Biochem. 93, 213–220 (1979).

    Article  CAS  Google Scholar 

  7. Nakanishi, S. et al. Nature 278, 423–427 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Noda, M. et al. Nature 295, 202–206 (1982).

    Article  ADS  CAS  Google Scholar 

  9. Gubler, U., Seeburg, P., Hoffman, B. J., Gage, L. P. & Udenfriend, S. Nature 295, 206–208 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Stern, A. S. et al. Proc. natn. Acad. Sci. U.S.A. 76, 6680–6683 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Kilpatrick, D. L., Jones, B. N., Kojima, K. & Udenfriend, S. Biochem. biophys. Res. Commun. 103, 698–705 (1981).

    Article  CAS  Google Scholar 

  12. Comb, M., Seeburg, P. H., Adelman, J., Eiden, L. & Herbert, E. Nature 295, 663–666 (1982).

    Article  ADS  CAS  Google Scholar 

  13. Noda, M. et al. Nature 297, 431–434 (1982).

    Article  ADS  CAS  Google Scholar 

  14. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982).

    Article  CAS  Google Scholar 

  15. Hirose, T., Crea, R. & Itakura, K. Tetrahedron Lett. 28, 2449–2452 (1978).

    Article  Google Scholar 

  16. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  17. Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Kangawa, K., Minamino, N., Chino, N., Sakakibara, S. & Matsuo, H. Biochem. biophys. Res. Commun. 99, 871–878 (1981).

    Article  CAS  Google Scholar 

  19. Maniatis, T., Kee, S. G., Efstratiadis, A. & Kafatos, F. C. Cell 8, 163–182 (1976).

    Article  CAS  Google Scholar 

  20. Efstratiadis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).

    Article  CAS  Google Scholar 

  21. Browne, J. K. et al. Science 195, 389–391 (1977).

    Article  ADS  CAS  Google Scholar 

  22. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).

    Article  CAS  Google Scholar 

  23. Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N.Y. Acad. Sci. 343, 1–16 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Dayhoff, M. D. in Atlas of Protein Sequence and Structure Vol. 5, 173–227 (National Biomedical Research Foundation, Washington DC, 1972).

    Google Scholar 

  25. Numa, S. & Nakanishi, S. Trends biochem. Sci. 6, 274–277 (1981).

    Article  CAS  Google Scholar 

  26. Grantham, R., Gautier, C., Gouy, M., Mercier, R. & Pavé, A. Nucleic Acids Res. 8, r49–r62 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  28. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  29. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  30. Curtiss, R. III et al. in Recombinant Molecules, Impact on Science and Society (eds Beers, R. F. & Bassett, E. G.) 45–56 (Raven, New York, 1977).

    Google Scholar 

  31. Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459–472 (1969).

    Article  CAS  Google Scholar 

  32. Wahl, G. M., Padgett, R. A. & Stark, G. R. J. biol. Chem. 254, 8679–8689 (1979).

    CAS  PubMed  Google Scholar 

  33. Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980).

    Article  CAS  Google Scholar 

  34. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).

    Article  CAS  Google Scholar 

  35. Reddy, V. B. et al. Science 200, 494–502 (1978).

    Article  ADS  CAS  Google Scholar 

  36. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).

    Article  ADS  CAS  Google Scholar 

  37. Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  38. Weinstock, R., Sweet, R., Weiss, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kakidani, H., Furutani, Y., Takahashi, H. et al. Cloning and sequence analysis of cDNA for porcine β-neo-endorphin/dynorphin precursor. Nature 298, 245–249 (1982). https://doi.org/10.1038/298245a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/298245a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing