Abstract
Apamin1 is a neurotoxic polypeptide of known structure2,3 isolated from bee venom. Shuba and coworkers4,5 have recently shown that it abolishes the hyperpolarising action of externally-applied ATP on visceral smooth muscle (guinea pig stomach and taenia coli) as well as the hyperpolarisation (inhibitory Junction potential) that follows stimulation of the non-adrenergic inhibitory nerve supply to these tissues. As it has been proposed6 that ATP is the neurotransmitter involved in the latter response, Vladimirova and Shuba4 tentatively concluded that apamin is a specific postsynaptic blocking agent of this non-adrenergic, possibly ‘purinergic’, inhibition. We have confirmed the important observation that nanomolar concentrations of apamin reduce inhibition by ATP and by non-adrenergic nerve stimulation, but further experiments suggest that, rather than acting as a specific blocker of ATP receptors, apamin inhibits the increase in potassium permeability caused by a number of agents, including ATP.
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Banks, B., Brown, C., Burgess, G. et al. Apamin blocks certain neurotransmitter-induced increases in potassium permeability. Nature 282, 415–417 (1979). https://doi.org/10.1038/282415a0
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DOI: https://doi.org/10.1038/282415a0
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