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Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells

Nature volume 265, pages 69–72 (1977)Cite this article

An Erratum to this article was published on 01 January 1977

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Abstract

OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets1. Cytoplasmic macromolecules2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects3–6. It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response7. But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process8–12 which is saturable and temperature-dependent13–17. We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.

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Change history

  • 01 January 1977

    In the article 'Specific bindin sites for oestrogen at the outer surfaces of isolated endometrial cells' by Richard J. Pietras and Clara M. Szego (Nature, 265, 71; 1977), the ordinate labels to Fig. 3 should read from 0 to 80, not from 10 to 90.

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Authors and Affiliations

  1. Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California, 90024

    RICHARD J. PIETRAS & CLARA M. SZEGO

Authors
  1. RICHARD J. PIETRAS
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  2. CLARA M. SZEGO
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PIETRAS, R., SZEGO, C. Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells. Nature 265, 69–72 (1977). https://doi.org/10.1038/265069a0

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