Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Abstract

Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Sarabhai, A. S., Stretton, A. O. W., Brenner, S., and Bolle, A., Nature, 201, 13 (1964).

    Article  ADS  CAS  Google Scholar 

  2. Kellenberger, E., Virology, 34, 549 (1968).

    Article  CAS  Google Scholar 

  3. Baylor, M. B., and Roslansky, P. F., Virology, 40, 251 (1970).

    Article  CAS  Google Scholar 

  4. Epstein, R. H., Bolle, A., Steinberg, C. H., Kellenberg, E., Boy de la Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., and Lielausis, J., Cold Spring Harbor Symp. Quant. Biol., 28, 375 (1963).

    Article  CAS  Google Scholar 

  5. Laemmli, U. K., Molbert, E., Showe, M., and Kellenberger, E., J. Mol. Biol., 49, 99 (1970).

    Article  CAS  Google Scholar 

  6. Laemmli, U. K., Beguin, F., and Gujer-Kellenberger, G., J. Mol. Biol., 47, 69 (1970).

    Article  CAS  Google Scholar 

  7. Edgar, R. S., and Wood, W. B., Proc. US Nat. Acad. Sci., 55, 498 (1966).

    Article  ADS  CAS  Google Scholar 

  8. Davis, B. J., Ann. NY Acad. Sci., 121, 404 (1964).

    Article  ADS  CAS  Google Scholar 

  9. Maizel, J. V., Fundamental Techniques of Virology (edit. by Habel, K., and Salzman, N. P.), chap. 32, 334 (Academic Press, New York, 1969).

    Google Scholar 

  10. Shapiro, A. L., Viñuela, E., and Maizel, J. V., Biophys. Biochem. Res. Commun., 28, 815 (1967).

    Article  CAS  Google Scholar 

  11. Minigawa, T., Virology, 13, 515 (1961).

    Article  Google Scholar 

  12. Hosoda, J., and Levinthal, C., Virology, 34, 709 (1968).

    Article  CAS  Google Scholar 

  13. King, J., J. Mol. Biol., 32, 231 (1968).

    Article  CAS  Google Scholar 

  14. King, J., FEBS Symp. (edit. by Ochoa, S., Nachmansohn, D., Asensio, C., and Heredia, C. F.), 21, 156 (Academic Press, London, 1970).

    Google Scholar 

  15. Favre, R., Boy de la Tour, E., Segre, N., and Kellenberger, E., J. Ultrastruct. Res., 13, 318 (1965).

    Article  CAS  Google Scholar 

  16. Kellenberger, E., Eiserling, F. A., and Boy de la Tour, E., J. Ultrastruct. Res., 21, 335 (1968).

    Article  Google Scholar 

  17. Eddleman, H. L., and Champe, S. P., Virology, 30, 471 (1966).

    Article  CAS  Google Scholar 

  18. Sternberg, N., and Champe, S. P., J. Mol. Biol., 46, 337 (1970).

    Google Scholar 

  19. Laemmli, U. K., and Eiserling, F. A., Molec. Gen. Genet., 101, 333 (1968).

    Article  CAS  Google Scholar 

  20. Fairbanks, G., Levinthal, C., and Reeder, R. H., Biochem. Biophys. Res. Commun., 20, 393 (1965).

    Article  CAS  Google Scholar 

  21. Larcom, L., Bendet, I., and Mumma, S., Virology, 41, 1 (1970).

    Article  CAS  Google Scholar 

  22. Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

LAEMMLI, U. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature 227, 680–685 (1970). https://doi.org/10.1038/227680a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/227680a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing