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N-cadherin involvement in the heterotypic adherence of malignant T-cells to epithelia

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Abstract

N-cadherin, a cell adhesion molecule normally found in neural cell tissue, has been found recently to be expressed on the surface of malignant T-cells. The function of N-cadherin on these cells remains unclear. Heterotypic assays between Molt-3 T lymphoblastic leukemia cells and Caco-2 epithelial monolayers were examined under different conditions to assess the functional role of N-cadherin. The results indicate that adherence of Molt-3 cells to Caco-2 monolayers was reduced significantly following pretreatment of Molt-3 cells with 100 μM of an N-cadherin-derived antagonist decapeptide. In contrast, pretreatment of Molt-3 cells with an anti-N-cadherin antibody raised against the first 20 amino acids of N-cadherin sequence led to a surprisingly marked enhancement of Molt-3 cell adherence to Caco-2 monolayers. In addition, the presence of anti-N-cadherin antibody neutralized the inhibitory effect of anti-ICAM-1 on Molt-3 adhesion to Caco-2 monolayers. This novel finding demonstrates that external stimulus through the N-cadherin amino terminus can modulate adhesion of malignant T-cells to epithelia and may promote their ability to invade or metastasize to inflammatory sites.

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Authors
  1. Irwan T. Makagiansar
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  2. Helena Yusuf-Makagiansar
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  3. Atsutoshi Ikesue
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  4. Anna M. Calcagno
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  5. Joseph S. Murray
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  6. Teruna J. Siahaan
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Makagiansar, I.T., Yusuf-Makagiansar, H., Ikesue, A. et al. N-cadherin involvement in the heterotypic adherence of malignant T-cells to epithelia. Mol Cell Biochem 233, 1–8 (2002). https://doi.org/10.1023/A:1015556625038

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  • DOI: https://doi.org/10.1023/A:1015556625038

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