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Enhanced Oral Uptake of Tomato Lectin-Conjugated Nanoparticles in the Rat

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Abstract

Purpose. To investigate the usefulness of a surface-conjugated, bioadhesive molecule, tomato lectin, to augment intestinal uptake of orally administered inert nanoparticles.

Methods. Fluorescent 500 nm polystyrene nanoparticles with tomato lectin covalently surface coupled using a carbodiimide reaction were administered to female Wistar rats by oral gavage daily for 5 days.

Results. Analysis of tissue extracted polymer by gel permeation chromatography revealed a 23% systemic uptake of tomato lectin conjugated nanoparticles compared to < 0.5% of TL nanoparticles blocked with N-acetylchitotetraose thus representing an increase of almost 50 fold across the intestine. Intestinal uptake of tomato lectin-conjugated nanoparticles via the villous tissue was 15 times higher than uptake by the gut-associated lymphoid tissue.

Conclusions. The application of tomato lectin as a bioadhesive agent in vivo has been demonstrated to enhance subsequent intestinal transcytosis of colloidal particulates to which it is bound.

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Author notes

  1. Nasir Hussain

    Present address: Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee, 38101

Authors and Affiliations

  1. Centre for Drug Delivery Research, The School of Pharmacy, University of London, Brunswick Square, London, WC1N 1AX, United Kingdom

    Nasir Hussain, Praful U. Jani & Alexander T. Florence

Authors
  1. Nasir Hussain
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  2. Praful U. Jani
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  3. Alexander T. Florence
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Deceased December 2, 1995.

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Hussain, N., Jani, P.U. & Florence, A.T. Enhanced Oral Uptake of Tomato Lectin-Conjugated Nanoparticles in the Rat. Pharm Res 14, 613–618 (1997). https://doi.org/10.1023/A:1012153011884

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  • DOI: https://doi.org/10.1023/A:1012153011884

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