Skip to main content
SpringerLink
Log in

Role of Na+-Glucose Cotransport in Jejunal Meal-Induced Absorption

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

In awake dogs, meal ingestion stimulates the absorption of water and electrolytes from neurovascularly intact jejunal Thiry-Vella loops, even though these loops are isolated from the remainder of the gut. This study was designed to investigate the role of Na+–glucose cotransport in mediating this event. Meal ingestion enhanced absorption when the jejunal lumen was perfused with an isotonic solution containing d-glucose, d-galactose, or 3-O-methylglucose. This response was absent when the perfusate contained mannitol or when phlorizin was added to the d-glucose solution. Mucosa from the jejunal loops was serially biopsied and assayed for brush-border Na+-glucose cotransporter (SGLT1) mRNA and protein expression. Although no changes in SGLT1 mRNA levels were observed, protein levels significantly increased within 30 min following meal ingestion. The time course of SGLT1 protein expression corresponded with that of increased Na+ and water absorption. These results suggest that meal-stimulated jejunal absorption may be mediated through an induction of mucosal SGLT1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Ferraris RP, Diamond JM: Crypt/villus site of substrate-dependent regulation of mouse intestinal glucose transporters. Proc Natl Acad Sci USA. 90:5868-5872, 1993

    PubMed  Google Scholar 

  2. Burant CF, Flink S, DePaoli AM, Chen J, Lee WS, Hediger MA, Buse JB, Chang EB: Small intestinal hexose transport in experimental diabetes. J Clin Invest 93:578-585, 1994

    PubMed  Google Scholar 

  3. Secor SM, Stein ED, Diamond J: Rapid upregulation of snake intestine in response to feeding: A new model of intestinal adaptation. Am J Physiol 266(4 Pt 1):G695-G705, 1994

    PubMed  Google Scholar 

  4. Secor SM, Diamond JA: Vertebrate model of extreme physiological regulation. Nature 395:659-662, 1998

    PubMed  Google Scholar 

  5. Keren DF, Elliott HL, Brown GD, Yardley JH: Atrophy of villi with hypertrophy and hyperplasia of Paneth cells of isolated (Thiry-Vella) ileal loops in rabbits. Gastroenterology 68:83-93, 1975

    PubMed  Google Scholar 

  6. Bright-Asare P, Binder HJ: Stimulation of colonic secretion of water and electrolytes by hydroxy fatty acids. Gastroenterology 64:81-88, 1973

    PubMed  Google Scholar 

  7. Miller DL, Schedl HP: Total recovery studies of nonabsorbable indicator in the rat small intestine. Gastroenterology 58:40-46, 1970

    PubMed  Google Scholar 

  8. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159, 1987

    Article  PubMed  Google Scholar 

  9. Hediger MA, Coady MJ, Ikeda TS, Wright EM: Expression cloning and cDNA sequencing of the Na+-glucose cotransporter. Nature 330:379-391, 1987

    PubMed  Google Scholar 

  10. Gershoni JM, Palade GE: Protein blotting: principles and applications. Anal Biochem 131:1-15, 1983

    PubMed  Google Scholar 

  11. Hirayama BA, Wong HC, Smith CD, Hagenbuch BA, Hediger MA, Wright EM: Intestinal and renal Na+/glucose cotransporters share common structures. Am J Physiol 261(2 Pt 1):C296-C304, 1991

    PubMed  Google Scholar 

  12. Bastidas JA, Orandle MS, Zinner MJ, Yeo CJ: Small bowel origin of the signal for meal-induced jejunal absorption. Surgery 108:376-383, 1988

    Google Scholar 

  13. Sarr MG, Kelly KA, Phillips SF: Canine jejunal absorption and transit during interdigestive and digestive motor states. Am J Physiol 239:G402-G409, 1980

    Google Scholar 

  14. Yeo CJ, Bastidas JA, Schmieg RE, Zinner MJ: Meal-stimulated absorption of water and electrolytes in canine jejunum. Am J Physiol 259:G402-G409, 1990

    PubMed  Google Scholar 

  15. Moneta GL, Taylor DC, Helton WS, Mulholland MW, Strandness DE: Duplex ultrasound measurement of postprandial intestinal blood flow: effect of meal composition. Gastroenterology 95:1294-1301, 1988

    PubMed  Google Scholar 

  16. Yamada T: Gut hormone release induced by food ingestion. Am J Clin Nutr 42:1033-1039, 1985

    PubMed  Google Scholar 

  17. Hines OJ, Bilchik AJ, Rodgers PJ, Berger J, Zinner MJ, Ashley SW: Na+/H+ exchange regulates postprandial ileal absorption. Dig Dis Sci 40(4):774-780, 1995

    PubMed  Google Scholar 

  18. Maher MM, Gontarek JD, Jimenez RE, Donowitz M, Yeo CJ: Role of brush border Na+/H+ exchange in canine ileal absorption. Dig Dis Sci 41(4):651-659, 1996

    PubMed  Google Scholar 

  19. Csaky TZ, Fischer E: Intestinal sugar transport in experimental diabetes. Diabetes 30(7):568-574, 1981

    PubMed  Google Scholar 

  20. Pappenheimer JR, Reiss KZ: Contribution of solvent drag through intercellular junctions to absorption of nutrients by the small intestine of the rat. J Membr Biol 100:123-136, 1987

    PubMed  Google Scholar 

  21. Pappenheimer JR: Paracellular intestinal absorption of glucose, creatinine, and mannitol in normal animals: Relation to body size. Am J Physiol 259:G290-G299, 1990

    PubMed  Google Scholar 

  22. Fine KD, Santa Ana CA, Porter JL, Fordtran JA: Effect of D-glucose on intestinal permeability and its passive absorption in human small intestine in vivo. Gastroenterology 105:1117-1125, 1993

    PubMed  Google Scholar 

  23. Lane JS, Whang EE, Rigberg DA, Hines OJ, Zinner MJ, McFadden DW, Diamond J, Ashley SW: Paracellular glucose transport plays a minor role in the unanesthetized dog. Am J Physiol (in press)

  24. Cheeseman CI: Upregulation of SGLT-1 transport activity in rat jejunum induced by GLP-2 infusion in vivo. Am J Physiol 273(6 Pt 2):R1965-R1971, 1997

    PubMed  Google Scholar 

  25. Brubaker PL, Crivici A, Izzo A, Ehrlich P, Tsai CH, Drucker DJ: Circulating and tissue forms of the intestinal growth factor, glucagon-like peptide-2. Endocrinology 138(11):4837-4843, 1997

    PubMed  Google Scholar 

  26. Oishi AJ, Inoue Y, Souba WW, Sarr MG: Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation. Dig Dis Sci 41:1915-1924, 1996

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Surgery, UCLA School of Medicine, Los Angeles, California; and

    Oscar J. Hines, Edward E. Whang, Anton J. Bilchik, Michael J. Zinner, Mark L. Welton, John Lane, David W. McFadden & Stanley W. Ashley

  2. Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts

    Oscar J. Hines, Edward E. Whang, Anton J. Bilchik, Michael J. Zinner, Mark L. Welton, John Lane, David W. McFadden & Stanley W. Ashley

Authors
  1. Oscar J. Hines
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edward E. Whang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anton J. Bilchik
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael J. Zinner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mark L. Welton
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David W. McFadden
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Stanley W. Ashley
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hines, O.J., Whang, E.E., Bilchik, A.J. et al. Role of Na+-Glucose Cotransport in Jejunal Meal-Induced Absorption. Dig Dis Sci 45, 1–6 (2000). https://doi.org/10.1023/A:1005477204960

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005477204960

Search

Navigation