Characterization of the bombesin-like peptide receptor family in primates
Section snippets
Search for mammalian BB4R
RT-PCR and genomic PCR were conducted on rhesus, Xenopus, and human brain cDNA or genomic DNA using primers for the reported rhesus BB4R DNA fragment [10]. The primers were almost perfectly conserved with the Bombina BB4R DNA sequence. Unexpectedly, an amplified fragment from Xenopus cDNA and genomic DNA that perfectly matched the partial rhesus BB4R sequence [10] was found. Attempts to detect the partial rhesus BB4R by RT-PCR using human and rhesus mRNA samples were not successful. These
Poly(A)+ mRNA and genomic DNA isolation
Poly(A)+ mRNAs were isolated from rhesus hypothalamus, cerebellum, pituitary gland, pons, medulla oblongata, liver, brain (except for hypothalamus, cerebellum, pituitary gland, pons, medulla oblongata), liver, and testis using FastTrack 2.0 (Invitrogen, Carlsbad, CA, USA). Genomic DNA was purified from rhesus liver and Xenopus laevis brain. Human poly(A)+ RNAs and genomic DNA were purchased from Clontech (Palo Alto, CA, USA).
Genomic and RT-PCR for BB4R
Primers were based on a rhesus BB4 partial sequence [10] and had high
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Hypothalamic response with PKA/CREB signaling is associated with direct cerebroventricular administration of bombesin-induced scratching
2022, Brain ResearchCitation Excerpt :Moreover, our results establishing the distribution of GRPRs and NMBRs in the hypothalamus suggest that it may play a critical role in the supraspinal circuits modulating BN-mediated itch. Consistent with previous reports (Battey and Wada, 1991; Kamichi et al., 2005; Pert et al., 1980; Sano et al., 2004), the distribution of GRPRs and NMBRs in the hypothalamus indicate that this brain area is specifically sensitive to BN, indicating a potential role in processing of itch sensation. Indeed, we detected a specific neuronal c-Fos response in the dorsomedial nucleus of the hypothalamus, which exhibited a dense BN receptor distribution (Fig. 4A), after i.c.v. BN administration (Fig. 4C).
Discovery of oridonin as a novel agonist for BRS-3
2022, PhytomedicineGastrin-releasing peptide facilitates glutamatergic transmission in the hippocampus and effectively prevents vascular dementia induced cognitive and synaptic plasticity deficits
2017, Experimental NeurologyCitation Excerpt :There is a possible reason that NMB and GRP are differentially distributed in the rat nervous system which suggests that these 2 structurally related peptides may have very distinct functions as neuropeptides in the rat nervous system (Moody et al., 1986). In addition, it has been recognized that NMBR has greater than a 100-food higher affinity for NMB than GRP, whereas the GRPR has a greater than 50-fold higher affinity for GRP than NMB (Jensen et al., 2008; Sano et al., 2004) which might be an explanation of more effective in improving learning and memory of NMB than GRP. And other studies also found that GRP and NMB could exert not exactly the same physiological action in anxiety and fear-related behavior (Bedard et al., 2007).