Claudin profiling in the mouse during postnatal intestinal development and along the gastrointestinal tract reveals complex expression patterns
Section snippets
Claudin expression patterns in the adult mouse intestine
Quantitative real-time PCR (qRT-PCR) reveals that the adult intestine expresses many different claudins in complex quantitative and spatial patterns. Claudins are expressed variably along both the longitudinal axis and the crypt/villus differentiation axis (Fig. 1, Fig. 3, Fig. 4 and Supplementary Fig. 3 and Table 1). In contrast, transcript levels for the TJ cytoplasmic protein ZO-1 do not vary relative to expression of Eef1a1, which was used as a general reference transcript. Therefore, in
Mice and tissue collection
All animal handling and tissue collection was preformed in accordance with the policies of the Institutional Animal Care Committees of the University of North Carolina-Chapel Hill. Full thickness bowel segments, stripped of omentum, were collected from female C57/Black6 mice 1, 14, 28, and 90 days after birth. For the 90 day adult time-point, samples for RNA isolation and immunofluorescent microscopic localization (IF) were taken from the duodenum, mid-jejunum, mid-ileum, cecum (the cecal tip
Acknowledgments
This work is supported by the National Institutes of Health (NIDDK DK45134), the Broad Medical Research Program and the State of North Carolina. We thank Alan Fanning for helpful discussions.
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