Basic Science and Experimental StudiesProteomic Analysis Reveals Significant Alternations of Cardiac Small Heat Shock Protein Expression in Congestive Heart Failure
Section snippets
Animal Preparation
All animal experiments were conducted according to the Guide for the Care and Use of Laboratory Animals (Department of Health and Human Services, National Institutes of Health, Publication No. 86-23) and to the Guidelines for Animal Experimentation at the Animal Research Committee of Shiga University of Medical Science. CHF was induced by rapid right ventricular pacing (240 ppm, 28 days) in 6 beagle dogs (CHF group), and a thermodilution catheter was implanted for measurement of cardiac output
Cardiohemodynamics and Blood Analysis
The results of cardiohemodynamics and blood analysis are summarized in Table 1. After 4 weeks of pacing, cardiac output significantly decreased and both LV end-diastolic pressure and pulmonary capillary wedge pressure increased compared to the normal group. LV end-diastolic dimensions increased and LV dP/dT max and LV% fractional shortening decreased compared with the normal group. Plasma norepinephrine levels were significantly higher in the CHF group than in the normal group. These data
Discussion
Using proteomic analysis, we first carried out screening of the protein changes in our paced dog model. Quantitative computer analysis of differentially expressed cardiac proteins showed as many as 500 cardiac protein spots. A total of 20 spots (14 increased, 6 decreased) was altered in CHF and 9 more distinguishably changed protein spots (6 increased, 3 decreased) were identified by using MALDI-TOF MS. The highly expressed spots in CHF were alpha B crystallin, HSP27, and HSP20, which maintain
Acknowledgments
We would like to thank Akiko Yoshii (Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science, Shiga, Japan), Noboru Urushiyama, and Masafumi Suzaki (Central Research Laboratory, Shiga University of Medical Science, Shiga, Japan) for their technical assistance.
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Supported by a grant-in-aid for Scientific Research (C) and a grant-in-aid for Encouragement of Young Scientists from the Minister of Education, Culture, Sports, Science and Technology of Japan.