Archival ReportDifferential Role of Anandamide and 2-Arachidonoylglycerol in Memory and Anxiety-like Responses
Section snippets
Animals
Male Swiss albino and C57BL/6J mice (Charles River, Lyon, France) and CB1 (16) and CB2 (Jackson Laboratory, Bar Harbor, Massachusetts) constitutive knockout mice (8–10 weeks of age), weighing 26 g to 30 g at the beginning of the experiments were used. Mice were housed five per cage in a temperature (21 ± 1°C) and humidity (55 ± 10%) controlled environment. Food and water were available ad libitum. All the experiments were performed during the light phase of a 12-hour light/dark cycle (lights on
Differential Effects of URB597 and JZL184 in Hippocampal Memory and Hippocampal Mammalian Target of Rapamycin Regulation
We used the object-recognition and context-recognition tasks to evaluate the effects of URB597 and JZL184 on memory consolidation. Using these paradigms, robust hippocampal-dependent learning can be achieved in a single training session (19, 21); therefore, memory consolidation can be modified pharmacologically afterward, precluding the possible influence of the acute pharmacologic effects of the drugs on locomotion or exploration during the memory acquisition or retrieval periods. Systemic
Discussion
The modulation of the endocannabinoid system is an emerging therapeutic approach, based on the well-demonstrated medicinal properties of compounds acting on this system (25). Selective and efficacious inhibitors of the two main enzymes involved in the catabolism of AEA and 2-AG, FAAH and MAGL, respectively, have been developed, allowing the selective increase of endocannabinoid concentrations in vivo (6, 7). We took advantage of these pharmacologic tools to modulate the endogenous levels of AEA
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Authors AB-G and EP contributed equally to this work.