Biochemical and Biophysical Research Communications
PGC-1α over-expression promotes recovery from mitochondrial dysfunction and cell injury
Section snippets
Materials and methods
Reagents. The primary antibodies used are as follows: ATP Synthaseβ (Abcam), ND6 (Molecular Probes), and GAPDH (Fitzgerald Antibodies and Antigens). All primary antibodies were used at 1:1000 dilutions. All other chemicals were purchased from Sigma (St. Louis, MO).
Isolation and culture of renal proximal tubules. Female New Zealand White rabbits (2 kg) were purchased from Myrtle’s Rabbitry (Thompson Station, TN). Procedures for the isolation of RPTC were approved by an institutional animal care
Over-expression of PGC-1α potentiates oxidant-induced mitochondrial dysfunction
Confluent RPTC were infected with adenovirus expressing either GFP of PGC-1α and then grown in culture for an additional 48 h. Over-expression of PGC-1α in RPTC for this time period resulted in significant increases in mitochondrial protein expression, and cellular respiration (Fig. 1) [9], revealing that over-expression of PGC-1α results in the biogenesis of functional mitochondria. RPTC were acutely exposed to TBHP (200 μM) or H2O2 (1 mM) for 30 min or 5 h, respectively, at which point media was
Acknowledgments
We thank Dr. Bruce Spiegelman (Harvard Medical School) for the generous gift of the adenoviral constructs used to express PGC-1α and GFP in these experiments. Work was performed with funding from National Institutes of Health Training Grant T32 HL007260.
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