Biochemical and Biophysical Research Communications
LTB4 stimulates growth of human pancreatic cancer cells via MAPK and PI-3 kinase pathways
Section snippets
Materials and methods
Materials. MEM, penicillin–streptomycin solution, and trypsin–EDTA solution were purchased from Sigma Chemicals (St. Louis, MO). Fetal bovine serum (FBS) was purchased from Altanta Biologicals (Norcross, GA). LTB4 was purchased from Cayman Chemicals (Ann Arbor, MI). The LTB4 receptor antagonist, LY293111, was from the Eli Lilly Research Laboratories (Indianapolis, IN). The MEK1/2 inhibitor, PD098059, was purchased from Calbiochem/Novabiochem International (La Jolla, CA). The MEK inhibitor,
Results and discussion
Our previous studies have demonstrated the expression of both LTB4 receptor sub-types, BLT1 and BLT2, in multiple human pancreatic cancer cells [28], [29]. The selective LTB4 receptor antagonist, LY293111, inhibited proliferation and induced apoptosis in these cells both in vitro and in vivo [29], [30]. In the current studies, we investigated the effect of LTB4 on the proliferation of pancreatic cancer cells and the possible mechanisms involved. The effect of LTB4 on human pancreatic cancer
Acknowledgments
These studies were supported by grants from the NCI SPORE program (P50 CA72712) and the Lustgarten Foundation for Pancreatic Cancer Research. The selective LTB4 receptor antagonist, LY293111, was a kind gift from Drs. Jake Starling and Jerome Fleisch, Eli Lilly Research Laboratories (Indianapolis, IN).
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